大肠杆菌基因组中胸腺嘧啶碱基被修饰碱基替代。
Replacement of Thymidine by a Modified Base in the Escherichia coli Genome.
机构信息
The Scripps Research Institute , 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
出版信息
J Am Chem Soc. 2016 Jun 15;138(23):7272-5. doi: 10.1021/jacs.6b03904. Epub 2016 Jun 3.
Abstract
Prokaryotic and eukaryotic genomic DNA is comprised of the four building blocks A, G, C, and T. We have begun to explore the consequences of replacing a large fraction or all of a nucleoside in genomic DNA with a modified nucleoside. As a first step we have investigated the possibility of replacement of T by 2'-deoxy-5-(hydroxymethyl)uridine (5hmU) in the genomic DNA of Escherichia coli. Metabolic engineering with phage genes followed by random mutagenesis enabled us to achieve approximately 75% replacement of T by 5hmU in the E. coli genome and in plasmids.
摘要
原核生物和真核生物的基因组 DNA 由四个碱基 A、G、C 和 T 组成。我们已经开始探索用修饰碱基替代基因组 DNA 中大量或全部核苷的后果。作为第一步，我们研究了用 2'-脱氧-5-(羟甲基)尿嘧啶（5hmU）替代大肠杆菌基因组 DNA 中 T 的可能性。噬菌体基因的代谢工程和随机诱变使我们能够在大肠杆菌基因组和质粒中实现大约 75%的 T 被 5hmU 替代。
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