Antonovsky Niv, Gleizer Shmuel, Noor Elad, Zohar Yehudit, Herz Elad, Barenholz Uri, Zelcbuch Lior, Amram Shira, Wides Aryeh, Tepper Naama, Davidi Dan, Bar-On Yinon, Bareia Tasneem, Wernick David G, Shani Ido, Malitsky Sergey, Jona Ghil, Bar-Even Arren, Milo Ron
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel.
Department of Computer Science, Technion, Israel Institute of Technology, Haifa 3200003, Israel.
Cell. 2016 Jun 30;166(1):115-25. doi: 10.1016/j.cell.2016.05.064. Epub 2016 Jun 23.
Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PAPERCLIP.
异养生物能否进化为直接从二氧化碳合成生物质？到目前为止，仅从二氧化碳合成生物质前体的非天然碳固定仍然是一个难以实现的巨大挑战。在这里，我们展示了合理的代谢重布线、重组表达和实验室进化相结合如何导致大肠杆菌中通过完整功能的卡尔文-本森-巴斯姆（CBB）循环生物合成糖类和其他主要生物质成分。在进化的细菌中，碳固定通过非天然的CBB循环进行，而还原力和能量则通过氧化供应的有机化合物（例如丙酮酸）获得。基因组测序表明，连接非天然CBB循环与生物合成途径的通量分支点处的突变对于这种表型至关重要。非天然碳固定途径的成功进化，尽管尚未导致净碳增益，但显著证明了适用于生物技术的代谢快速营养模式进化的能力。回形针。
