Hahn Alexander, Parey Kristian, Bublitz Maike, Mills Deryck J, Zickermann Volker, Vonck Janet, Kühlbrandt Werner, Meier Thomas
Department of Structural Biology, Max Planck Institute of Biophysics, Max-von-Laue-Str. 3, 60438 Frankfurt am Main, Germany.
Institute of Biochemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany.
Mol Cell. 2016 Aug 4;63(3):445-56. doi: 10.1016/j.molcel.2016.05.037. Epub 2016 Jun 30.
We determined the structure of a complete, dimeric F1Fo-ATP synthase from yeast Yarrowia lipolytica mitochondria by a combination of cryo-EM and X-ray crystallography. The final structure resolves 58 of the 60 dimer subunits. Horizontal helices of subunit a in Fo wrap around the c-ring rotor, and a total of six vertical helices assigned to subunits a, b, f, i, and 8 span the membrane. Subunit 8 (A6L in human) is an evolutionary derivative of the bacterial b subunit. On the lumenal membrane surface, subunit f establishes direct contact between the two monomers. Comparison with a cryo-EM map of the F1Fo monomer identifies subunits e and g at the lateral dimer interface. They do not form dimer contacts but enable dimer formation by inducing a strong membrane curvature of ∼100°. Our structure explains the structural basis of cristae formation in mitochondria, a landmark signature of eukaryotic cell morphology.
我们通过冷冻电镜和X射线晶体学相结合的方法，确定了来自解脂耶氏酵母线粒体的完整二聚体F1Fo - ATP合酶的结构。最终结构解析出了60个二聚体亚基中的58个。Fo中a亚基的水平螺旋围绕c环转子，总共6个分配给a、b、f、i和8亚基的垂直螺旋跨越膜。8亚基（人类中的A6L）是细菌b亚基的进化衍生物。在膜内腔表面，f亚基在两个单体之间建立直接接触。与F1Fo单体的冷冻电镜图谱比较，在二聚体侧向界面处鉴定出e和g亚基。它们不形成二聚体接触，但通过诱导约100°的强膜曲率来促进二聚体形成。我们的结构解释了线粒体中嵴形成的结构基础，这是真核细胞形态的标志性特征。
