Han Geon Goo, Kim Eun Bae, Lee Jinyoung, Lee Jun-Yeong, Jin Gwideuk, Park Jongbin, Huh Chul-Sung, Kwon Ill-Kyong, Kil Dong Yong, Choi Yun-Jaie, Kong Changsu
Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.
Department of Animal Life Science, Kangwon National University, Chuncheon, Gangwon-do Republic of Korea.
Springerplus. 2016 Jun 29;5(1):911. doi: 10.1186/s40064-016-2604-8. eCollection 2016.
In the poultry industry, many efforts have been undertaken to further improve the growth performance of broilers and identification and modulation of body weight (BW)-related bacteria could be one of the strategies to improve productivity. However, studies regarding the relationship between microbiota and BW are scarce. The objective of the present study was to investigate the relationship between microbiota and BW in different sections of the gastrointestinal tract (GIT). A total of twenty 18-day-old birds were selected based on the BW, and samples were collected from the three different sections of the GIT, which included the crop, ileum and cecum. Bacterial genomic DNA was extracted from the samples, and the V4 region of 16S rRNA gene were amplified. Amplicons were sequenced on Illumina MiSeq, and microbial communities were analyzed by using QIIME. In principal coordinate analysis, bacterial communities were clustered into three groups, based on the sections of GIT. Several BW-related bacterial groups were identified from linear regression analysis. At the genus level, Streptococcus from the ileum as well as Akkermansia in both ileum and cecum, were negatively related to BW, whereas Bifidobacterium in the ileum and Lactococcus in the cecum showed a positive correlation. The results from the present study showed that particular bacterial communities in the GIT were related to BW, and the study has broadened the understanding of the intestinal microbial ecosystem in broiler chickens.
在家禽行业，人们已经做出了许多努力来进一步提高肉鸡的生长性能，识别和调节与体重（BW）相关的细菌可能是提高生产力的策略之一。然而，关于微生物群与体重之间关系的研究却很少。本研究的目的是调查胃肠道（GIT）不同部位的微生物群与体重之间的关系。根据体重选择了20只18日龄的鸡，并从GIT的三个不同部位采集样本，包括嗉囊、回肠和盲肠。从样本中提取细菌基因组DNA，并扩增16S rRNA基因的V4区域。扩增子在Illumina MiSeq上进行测序，并使用QIIME分析微生物群落。在主坐标分析中，细菌群落根据GIT的部位聚类为三组。通过线性回归分析确定了几个与体重相关的细菌群。在属水平上，回肠中的链球菌以及回肠和盲肠中的阿克曼氏菌与体重呈负相关，而回肠中的双歧杆菌和盲肠中的乳球菌呈正相关。本研究结果表明，GIT中特定的细菌群落与体重有关，该研究拓宽了对肉鸡肠道微生物生态系统的认识。
