Haslam Richard P, Sayanova Olga, Kim Hae Jin, Cahoon Edgar B, Napier Johnathan A
Biological Chemistry and Crop Protection, Rothamsted Research, West Common, Harpenden, AL5 2JQ, UK.
Centre for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Plant J. 2016 Jul;87(1):76-86. doi: 10.1111/tpj.13172. Epub 2016 Jun 20.
Plant seed lipid metabolism is an area of intensive research, including many examples of transgenic events in which oil composition has been modified. In the selected examples described in this review, progress towards the predictive manipulation of metabolism and the reconstitution of desired traits in a non-native host is considered. The advantages of a particular oilseed crop, Camelina sativa, as a flexible and utilitarian chassis for advanced metabolic engineering and applied synthetic biology are considered, as are the issues that still represent gaps in our ability to predictably alter plant lipid biosynthesis. Opportunities to deliver useful bio-based products via transgenic plants are described, some of which represent the most complex genetic engineering in plants to date. Future prospects are considered, with a focus on the desire to transition to more (computationally) directed manipulations of metabolism.
植物种子脂质代谢是一个深入研究的领域，包括许多转基因事件的实例，其中油脂成分已被改变。在本综述中描述的选定实例中，考虑了在非天然宿主中对代谢进行预测性操纵以及重建所需性状方面的进展。人们考虑了一种特殊的油料作物——亚麻荠作为先进代谢工程和应用合成生物学的灵活且实用的底盘的优势，以及在可预测地改变植物脂质生物合成能力方面仍然存在差距的问题。描述了通过转基因植物提供有用生物基产品的机会，其中一些代表了迄今为止植物中最复杂的基因工程。考虑了未来前景，重点是转向对代谢进行更多（计算上）定向操纵的愿望。
