University of Kentucky, Department of Biomedical Engineering, 143 Graham Avenue, Lexington, Kentucky 40506, United States.
University of Kentucky, Department of Biomedical Engineering, 143 Graham Avenue, Lexington, Kentucky 40506, United StatesbGwangju Institute of Science and Technology, Department of Biomedical Science and Engineering, 123 Cheomdan Gwagiro, Bukgu, Gwangju 6.
J Biomed Opt. 2016 Aug 1;21(8):80501. doi: 10.1117/1.JBO.21.8.080501.
We report a low-cost compact diffuse speckle contrast flowmeter (DSCF) consisting of a small laser diode and a bare charge-coupled-device (CCD) chip, which can be used for contact measurements of blood flow variations in relatively deep tissues (up to ∼ 8 mm). Measurements of large flow variations by the contact DSCF probe are compared to a noncontact CCD-based diffuse speckle contrast spectroscopy and a standard contact diffuse correlation spectroscopy in tissue phantoms and a human forearm. Bland–Altman analysis shows no significant bias with good limits of agreement among these measurements: 96.5%±2.2% (94.4% to 100.0%) in phantom experiments and 92.8% in the forearm test. The relatively lower limit of agreement observed in the in vivo measurements (92.8%) is likely due to heterogeneous reactive responses of blood flow in different regions/volumes of the forearm tissues measured by different probes. The low-cost compact DSCF device holds great potential to be broadly used for continuous and longitudinal monitoring of blood flow alterations in ischemic/hypoxic tissues, which are usually associated with various vascular diseases.
我们报告了一种低成本紧凑型漫散射斑纹对比血流仪（DSCF），它由一个小型激光二极管和一个裸露的电荷耦合器件（CCD）芯片组成，可用于相对深层组织（可达约 8 mm）的血流变化的接触测量。通过接触 DSCF 探头对大的血流变化的测量与非接触 CCD 基漫散射斑纹对比光谱学和组织体模和人体前臂中的标准接触漫散相关光谱学进行了比较。Bland–Altman 分析显示，这些测量之间没有显著的偏差，并且具有良好的一致性限制：在体模实验中为 96.5%±2.2%（94.4%至 100.0%），在前臂测试中为 92.8%。在体内测量中观察到的相对较低的一致性限制（92.8%）可能是由于不同探头测量的前臂组织的不同区域/体积的血流反应性不同所致。这种低成本紧凑型 DSCF 设备具有广泛用于监测缺血/缺氧组织中血流变化的潜力，这些组织通常与各种血管疾病有关。
