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用于血清中无标记和生物共轭识别DNA传感的共轭聚合物纳米颗粒

Conjugated Polymer Nanoparticles for Label-Free and Bioconjugate-Recognized DNA Sensing in Serum.

作者信息

Bao Biqing, Ma Mingfeng, Zai Huafeng, Zhang Lei, Fu Nina, Huang Wei, Wang Lianhui

机构信息

Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials Nanjing University of Posts and Telecommunications Nanjing 210023 Jiangsu P.R. China.

Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing Tech University Nanjing 211816 Jiangsu P.R. China.

出版信息

Adv Sci (Weinh). 2015 Feb 19;2(3):1400009. doi: 10.1002/advs.201400009. eCollection 2015 Mar.

DOI:10.1002/advs.201400009
PMID:27668149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5024028/
Abstract

Hybridbio/-synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label-free detection of target ssDNA in serum. Carboxylic acid-functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can significantly improve the photostability of the DNA-bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA-bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable fluorescent color for DNA-bioconjugated CPNs in the presence of target ssDNA allows naked-eye detection of ssDNA under UV irradiation.

摘要

杂化生物/合成的传感共轭聚合物纳米颗粒（CPNs）被开发用于血清中目标单链DNA（ssDNA）的选择性无标记检测。羧酸功能化的阴离子聚芴纳米颗粒被合理设计为信号放大单元，以与胺功能化的单链寡核苷酸作为受体进行生物共轭。在广泛的缓冲条件下，共价DNA涂层可以显著提高DNA共轭CPNs的光稳定性。由于纳米颗粒中链间相互作用增加和激子传输更有效，DNA共轭CPNs传感器对ssDNA有更好的区分能力。在目标ssDNA存在下，DNA共轭CPNs可区分的荧光颜色使得在紫外线照射下能够肉眼检测ssDNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/362ec925eeee/ADVS-2-0a-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/0e16e67954cc/ADVS-2-0a-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/11cb0c65b9ec/ADVS-2-0a-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/7f8efffac4a7/ADVS-2-0a-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/2fc156a91820/ADVS-2-0a-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/9a480be844f7/ADVS-2-0a-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/edee332a4f65/ADVS-2-0a-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/362ec925eeee/ADVS-2-0a-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/0e16e67954cc/ADVS-2-0a-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/11cb0c65b9ec/ADVS-2-0a-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/7f8efffac4a7/ADVS-2-0a-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/2fc156a91820/ADVS-2-0a-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/9a480be844f7/ADVS-2-0a-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/edee332a4f65/ADVS-2-0a-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56b5/5115288/362ec925eeee/ADVS-2-0a-g003.jpg

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