Jezierska Sylwia, Van Bogaert Inge N A
Laboratory for Industrial Biotechnology and Biocatalysis, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium.
J Ind Microbiol Biotechnol. 2017 May;44(4-5):721-733. doi: 10.1007/s10295-016-1858-z. Epub 2016 Nov 11.
How small molecules cross cellular membranes is an often overlooked issue in an industrial microbiology and biotechnology context. This is to a large extent governed by the technical difficulties to study these transport systems or by the lack of knowledge on suitable efflux pumps. This review emphasizes the importance of microbial cellular membranes in industrial biotechnology by highlighting successful strategies of membrane engineering towards more resistant and hence better performing microorganisms, as well as transporter and other engineering strategies for increased efflux of primary and secondary metabolites. Furthermore, the benefits and limitations of eukaryotic subcellular compartmentalization are discussed, as well as the biotechnological potential of membrane vesicles.
在工业微生物学和生物技术领域，小分子如何穿过细胞膜是一个常常被忽视的问题。这在很大程度上受限于研究这些转运系统的技术困难，或者缺乏关于合适外排泵的知识。本综述强调了微生物细胞膜在工业生物技术中的重要性，重点介绍了膜工程的成功策略，这些策略可使微生物更具抗性，从而表现更佳，还介绍了用于增加初级和次级代谢产物外排的转运体及其他工程策略。此外，还讨论了真核亚细胞区室化的益处和局限性，以及膜泡的生物技术潜力。
