Khalil Sabreen, El-Badri Nagwa, El-Mokhtaar Mohamed, Al-Mofty Saif, Farghaly Mohamed, Ayman Radwa, Habib Dina, Mousa Noha
Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt.
University of Science and Technology, Zewail City of Science and Technology, Giza, Egypt.
PLoS One. 2016 Dec 9;11(12):e0167116. doi: 10.1371/journal.pone.0167116. eCollection 2016.
We utilized the hAM to provide the biological and the three dimensional (3D) topographic components of the prototype. The 3D nano-roughness of the hAM was characterized using surface electron microscopy and surface image analysis (ImageJ and SurfaceJ). We developed additional macro-scale and micro-scale versions of the platform which provided additional shear stress factors to simulate the fluid dynamics of the in vivo extracellular fluids.
Three models of varying complexities of the prototype were assembled. A well-defined 3D surface modulation of the hAM in comparable to commercial 3D biomaterial culture substrates was achieved without complex fabrication and with significantly lower cost. Performance of the prototype was demonstrated through culture of primary human umbilical cord mononuclear blood cells (MNCs), human bone marrow mesenchymal stem cell line (hBMSC), and human breast cancer tissue.
This study presents methods of assembling an integrated, flexible and low cost biomimetic cell culture platform for diverse cell culture applications.
我们利用人羊膜提供原型的生物学和三维（3D）地形学成分。使用表面电子显微镜和表面图像分析（ImageJ和SurfaceJ）对人羊膜的3D纳米粗糙度进行表征。我们开发了该平台的额外宏观尺度和微观尺度版本，其提供了额外的剪切应力因素以模拟体内细胞外液的流体动力学。
组装了三种不同复杂程度的原型模型。在无需复杂制造且成本显著更低的情况下，实现了与人羊膜的3D表面调制明确可比的商业化3D生物材料培养底物。通过原代人脐带单个核血细胞（MNCs）、人骨髓间充质干细胞系（hBMSC）和人乳腺癌组织的培养，证明了该原型的性能。
本研究提出了用于多种细胞培养应用的组装集成、灵活且低成本的仿生细胞培养平台的方法。
