小鼠体长与眼部参数的关联
Association of Body Length with Ocular Parameters in Mice.
作者信息
Chakraborty Ranjay, Park Han Na, Tan Christopher C, Weiss Paul, Prunty Megan C, Pardue Machelle T
机构信息
*PhD †BS ‡MS Rehab R&D Center of Excellence, Atlanta VA Medical Center, Decatur (RC, CCT, MCP, MTP); Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta (MTP); Department of Ophthalmology, Emory University School of Medicine, Atlanta (RC, HP, CCT, MTP); and Department of Biostatistics and Bioinformatics, Emory University Rollins School of Public Health, Atlanta, Georgia (PW).
出版信息
Optom Vis Sci. 2017 Mar;94(3):387-394. doi: 10.1097/OPX.0000000000001036.
PURPOSE
To determine the association between changes in body length with ocular refraction, corneal radii, axial length, and lens thickness in two different mouse strains.
METHODS
Body length, ocular refraction, corneal radii, axial length, and lens thickness were measured for two inbred mouse strains: 129S1/SvJ (n = 7) and C57BL/6 J (n = 10) from 4 to 12 weeks of age. Body length, from tip of nose to base of tail, was obtained using a digital camera. Biometric parameters, corneal radii, and refractions were measured using spectral-domain optical coherence tomography, automated keratometry, and infrared photorefraction, respectively. A mixed-model ANOVA was performed to examine the changes in ocular parameters as a function of body length and strain in mice controlling for age, gender, and weight over time.
RESULTS
C57BL/6J mice had significantly longer body length (average body length at 10 weeks, 8.60 ± 0.06 cm) compared to 129S1/SvJ mice (8.31 ± 0.05 cm) during development (P < .001). C57BL/6J mice had significantly hyperopic refractions compared to 129S1/SvJ mice across age (mean refraction at 10 weeks, 129S1/SvJ: +0.99 ± 0.44D vs. C57BL/6J: +6.24 ± 0.38D, P < .001). Corneal radius of curvature, axial length, and lens thickness (except 10 weeks lens thickness) were similar between the two strains throughout the measurement. In the mixed-model ANOVA, changes in body length showed an independent and significant association with the changes in refraction (P = .002) and corneal radii (P = .016) for each mouse strain. No significant association was found between the changes in axial length (P = .925) or lens thickness (P = .973) as a function of body length and strain.
CONCLUSIONS
Changes in body length are significantly associated with the changes in ocular refraction and corneal radii in different mouse strains. Future studies are needed to determine if the association between body length and ocular refraction are related to changes in corneal curvature in mice.
目的
确定两种不同小鼠品系的体长变化与眼屈光、角膜半径、眼轴长度和晶状体厚度之间的关联。
方法
对两种近交系小鼠品系进行测量，分别为4至12周龄的129S1/SvJ（n = 7）和C57BL/6 J（n = 10）。使用数码相机测量从鼻尖到尾基部的体长。分别使用光谱域光学相干断层扫描、自动角膜曲率计和红外 photorefraction测量生物特征参数、角膜半径和屈光。进行混合模型方差分析，以检查在控制年龄、性别和体重随时间变化的情况下，小鼠眼参数随体长和品系的变化。
结果
在发育过程中，C57BL/6J小鼠的体长显著长于129S1/SvJ小鼠（10周龄时平均体长，C57BL/6J为8.60±0.06 cm，129S1/SvJ为8.31±0.05 cm，P <.001）。在各年龄段，C57BL/6J小鼠的屈光显著远视，与129S1/SvJ小鼠相比（10周龄时平均屈光，129S1/SvJ：+0.99±0.44D，C57BL/6J：+6.24±0.38D，P <.001）。在整个测量过程中，两种品系的角膜曲率半径、眼轴长度和晶状体厚度（10周龄时晶状体厚度除外）相似。在混合模型方差分析中，体长变化显示与每个小鼠品系的屈光变化（P =.002）和角膜半径变化（P =.016）存在独立且显著的关联。未发现眼轴长度变化（P =.925）或晶状体厚度变化（P =.973）与体长和品系之间存在显著关联。
结论
不同小鼠品系的体长变化与眼屈光和角膜半径变化显著相关。未来需要进行研究以确定体长与眼屈光之间的关联是否与小鼠角膜曲率变化有关。
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