Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Hezar Jerib Street, Isfahan, Iran.
Biomed Res Int. 2013;2013:349408. doi: 10.1155/2013/349408. Epub 2012 Dec 31.
Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.
细胞表面抗原作为生物标志物，在癌症等多种疾病的早期诊断、预后和治疗反应方面具有巨大的潜力。在这项研究中，应用了一种基于磁性纳米粒子和磁性细胞分离原理的简单、快速、准确、廉价且易于获得的体外检测方法，以鉴定和定量分析前列腺癌细胞的细胞表面抗原表达。将该检测方法的性能与流式细胞术这一金标准方法进行比较，结果显示出相似的结果。研究结果表明，用抗体包被的磁性纳米粒子进行抗原特异性磁细胞分离，对于定量细胞表面抗原检测和分析具有很大的潜力。
