Rana Ajay, Sharma Eshita, Rawat Kiran, Sharma Ranjana, Verma Sarika, Padwad Yogendra, Gulati Ashu
Food and Nutraceuticals Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061 India.
J Food Sci Technol. 2016 Nov;53(11):4023-4032. doi: 10.1007/s13197-016-2406-6. Epub 2016 Nov 26.
Comparative investigation of major phytoconstituents was performed from various parts of tea plant viz. apical bud, subtending 1st-5th leaf, stem, coarse leaves, flowers, fruits and roots. From the results of comparative RP-HPLC-DAD analysis it was found that underutilized tea parts especially coarse leaves, flowers and fruits contains abundant amount of phenolics (17.5%) and catechins (4-5%). From these underutilized tea plant parts the catechins were extracted and purified and then screened for their anticancer, immunomodulatory effect and antimicrobial activity against food borne pathogens. The results showed that tea fruit extract exhibited higher toxicity against oral cancer cells and also promotes proliferation of mice splenocytes. The results of antimicrobial studies revealed the inhibitory effect of these extracts against both gram positive and gram negative bacteria. These investigations clearly demonstrated that the underutilized tea plant parts could act as economical and sustainable bioresource of functionally active constituents which further lead to the development of new cost-effective nutraceuticals and other formulations.
对茶树的各个部位进行了主要植物成分的比较研究，这些部位包括顶芽、第一至第五片叶的叶腋、茎、粗叶、花、果实和根。通过比较反相高效液相色谱-二极管阵列检测（RP-HPLC-DAD）分析结果发现，未充分利用的茶叶部位，特别是粗叶、花和果实，含有大量的酚类物质（17.5%）和儿茶素（4-5%）。从这些未充分利用的茶树部位提取并纯化了儿茶素，然后对其进行抗癌、免疫调节作用以及对食源性病原体的抗菌活性筛选。结果表明，茶果提取物对口腔癌细胞具有更高的毒性，并且还能促进小鼠脾细胞的增殖。抗菌研究结果显示，这些提取物对革兰氏阳性菌和革兰氏阴性菌均有抑制作用。这些研究清楚地表明，未充分利用的茶树部位可作为具有功能活性成分的经济且可持续的生物资源，这进一步推动了新型经济高效营养保健品和其他制剂的开发。
