Yeo Jie Ming, Tse Vivian, Kung Judy, Lin Hiu Yu, Lee Yee Ting, Kwan Joseph, Yan Bryan P, Tse Gary
School of Medicine, Imperial College London, London.
Department of Physiology, McGill University, Montreal, Quebec.
J Basic Clin Physiol Pharmacol. 2017 May 1;28(3):191-200. doi: 10.1515/jbcpp-2016-0110.
Experimental models used in cardiovascular research range from cellular to whole heart preparations. Isolated whole hearts show higher levels of structural and functional integration than lower level models such as tissues or cellular fragments. Cardiovascular diseases are multi-factorial problems that are dependent on highly organized structures rather than on molecular or cellular components alone. This article first provides a general introduction on the animal models of cardiovascular diseases. It is followed by a detailed overview and a historical perspective of the different isolated heart systems with a particular focus on the Langendorff perfusion method for the study of cardiac arrhythmias. The choice of species, perfusion method, and perfusate composition are discussed in further detail with particular considerations of the theoretical and practical aspects of experimental settings.
心血管研究中使用的实验模型涵盖从细胞到全心脏制剂。与组织或细胞碎片等较低水平的模型相比，离体全心脏显示出更高水平的结构和功能整合。心血管疾病是多因素问题，依赖于高度组织化的结构，而不仅仅是分子或细胞成分。本文首先对心血管疾病的动物模型进行了一般性介绍。随后详细概述了不同的离体心脏系统，并从历史角度进行了阐述，特别关注用于研究心律失常的Langendorff灌注方法。还进一步详细讨论了物种选择、灌注方法和灌注液成分，并特别考虑了实验设置的理论和实际方面。
