Lopez-Huertas Eduardo, Fonolla Juristo
Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), 1, Profesor Albareda, Granada 18008, Spain.
BIOSEARCH LIFE S.A. 66, Camino de Purchil, Granada 18004, Spain.
Redox Biol. 2017 Apr;11:384-389. doi: 10.1016/j.redox.2016.12.014. Epub 2016 Dec 26.
Hydroxytyrosol (HT) is a main phenolic component of olive oil. In this study, we investigated the safety and effects produced by HT purified (99.5%) from olive mill waste. HT was administered at a daily dosage of 45mg for 8 weeks to volunteers with mild hyperlipidemia (n=14). We measured markers of cardiovascular disease risk, enzyme markers of several clinical conditions, hematology, antioxidant parameters, vitamins and minerals at baseline (T0), 4 weeks (T4) and 8 weeks (T8). The values obtained at T4 and T8 were compared with baseline. We found that the HT dose administered was safe and mostly did not influence markers of cardiovascular disease, blood lipids, inflammatory markers, liver or kidney functions and the electrolyte balance. Serum iron levels remained constant but a significant (P<0.05) decrease in ferritin at T4 and T8 was found. Serum folate and red blood cell folate levels were also reduced at T4 and T8. Finally, vitamin C increased by two-fold at T4 and T8 compared with levels at baseline. These results indicate a physiologically relevant antioxidant function for HT through increasing endogenous vitamin C levels.
羟基酪醇（HT）是橄榄油的主要酚类成分。在本研究中，我们调查了从橄榄果渣中纯化得到的（纯度为99.5%）HT的安全性和所产生的效应。以每日45毫克的剂量对14名轻度高脂血症志愿者给予HT，为期8周。我们在基线期（T0）、4周（T4）和8周（T8）时测量了心血管疾病风险标志物、几种临床状况的酶标志物、血液学指标、抗氧化参数、维生素和矿物质。将T4和T8时获得的值与基线值进行比较。我们发现，所给予的HT剂量是安全的，且大多不会影响心血管疾病标志物、血脂、炎症标志物、肝脏或肾脏功能以及电解质平衡。血清铁水平保持恒定，但在T4和T8时发现铁蛋白显著（P<0.05）降低。在T4和T8时血清叶酸和红细胞叶酸水平也有所降低。最后，与基线水平相比，T4和T8时维生素C增加了两倍。这些结果表明，HT通过提高内源性维生素C水平具有生理相关的抗氧化功能。
