Institute of Pharmacology, National Yang-Ming University, College of Medicine, Taipei, Taiwan.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Sci Rep. 2017 Jan 12;7:38763. doi: 10.1038/srep38763.
Uraria crinita is widely used as a popular folk drink; however, little is known about how the post-harvest operations affect the chemical composition and bioactivity of UC. We assessed three drying methods (Oven-drying, Air-drying, Sun-drying), as well as the Oven-drying temperature using metabolomics approaches and bioactivity assays. The samples processed at 40 degree show a greater effect on the levels of estrogen receptor-alpha activity and nuclear factor erythroid 2-related factor 2 activity, anti-oxidative activity, and cyclooxygenase-2 inhibition compared with the other samples. A multivariate analysis showed a clear separation between the 40 degree Oven-dried samples and the other samples, which is consistent with the results of bioactivity assay. These results are ascribed to at least two-fold increase in the concentrations of flavonoids, spatholosineside A and triterpenoids in the oven-dried samples compared with the other groups. The proposed Oven-drying method at 40 degree results in an improved quality of UC.
翻白草被广泛用作一种受欢迎的民间饮品；然而，对于收获后操作如何影响 UC 的化学成分和生物活性知之甚少。我们使用代谢组学方法和生物活性测定评估了三种干燥方法（烘箱干燥、空气干燥、阳光干燥）以及烘箱干燥温度。与其他样品相比，在 40 度下加工的样品对雌激素受体-α活性和核因子红细胞 2 相关因子 2 活性、抗氧化活性和环氧化酶-2 抑制的影响更大。多变量分析表明，40 度烘箱干燥样品与其他样品之间存在明显分离，这与生物活性测定结果一致。这些结果归因于与其他组相比，烘箱干燥样品中类黄酮、翻白草苷 A 和三萜的浓度至少增加了两倍。建议在 40 度下采用烘箱干燥方法可提高翻白草的质量。
