Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, College of Chemistry and Chemical Engineering, Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, Qingdao University, Shandong 266071, PR China.
Biosens Bioelectron. 2017 May 15;91:523-537. doi: 10.1016/j.bios.2017.01.011. Epub 2017 Jan 7.
Previously developed electrochemical biosensors with single-electric signal output are probably affected by intrinsic and extrinsic factors. In contrast, the ratiometric electrochemical biosensors (RECBSs) with dual-electric signal outputs have an intrinsic built-in correction to the effects from system or background electric signals, and therefore exhibit a significant potential to improve the accuracy and sensitivity in electrochemical sensing applications. In this review, we systematically summarize the fabrication strategies, sensing modes and analytical applications of RECBSs. First, the different fabrication strategies of RECBSs were introduced, referring to the analytes-induced single- and dual-dependent electrochemical signal strategies for RECBSs. Second, the different sensing modes of RECBSs were illustrated, such as differential pulse voltammetry, square wave voltammetry, cyclic voltammetry, alternating current voltammetry, electrochemiluminescence, and so forth. Third, the analytical applications of RECBSs were discussed based on the types of target analytes. Finally, the forthcoming development and future prospects in the research field of RECBSs were also highlighted.
先前开发的具有单一电信号输出的电化学生物传感器可能会受到内在和外在因素的影响。相比之下，具有双电信号输出的比率型电化学生物传感器 (RECBS) 对系统或背景电信号的影响具有内在的内置校正功能，因此在电化学生物传感应用中具有显著提高准确性和灵敏度的潜力。在这篇综述中，我们系统地总结了 RECBS 的制备策略、传感模式和分析应用。首先，介绍了 RECBS 的不同制备策略，包括分析物诱导的单一和双重依赖电化学信号策略。其次，说明了 RECBS 的不同传感模式，例如差分脉冲伏安法、方波伏安法、循环伏安法、交流伏安法、电化学发光等。第三，根据目标分析物的类型讨论了 RECBS 的分析应用。最后，还强调了 RECBS 研究领域的未来发展和前景。
