向 PrP 的原子结构进军。
Toward the Atomic Structure of PrP.
机构信息
Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, University of California, Los Angeles, California 90095.
Department of Neurology, Molecular Biology Institute (MBI), and Brain Research Institute (BRI), David Geffen School of Medicine, University of California, Los Angeles, California 90095.
出版信息
Cold Spring Harb Perspect Biol. 2017 Sep 1;9(9):a031336. doi: 10.1101/cshperspect.a031336.
Abstract
In this review, we detail our current knowledge of PrP structure on the basis of structural and computational studies. We discuss the progress toward an atomic resolution description of PrP and results from the broader field of amyloid studies that may further inform our knowledge of this structure. Moreover, we summarize work that investigates the role of PrP structure in its toxicity, transmissibility, and species specificity. We look forward to an atomic model of PrP, which is expected to bring diagnostics and/or therapeutics to the field of prion disease.
摘要
在这篇综述中，我们基于结构和计算研究详细介绍了我们目前对 PrP 结构的了解。我们讨论了朝着原子分辨率描述 PrP 取得的进展，以及来自淀粉样蛋白研究领域的可能进一步阐明我们对这种结构的认识的结果。此外，我们总结了研究 PrP 结构在其毒性、传染性和物种特异性中的作用的工作。我们期待着 PrP 的原子模型的出现，这有望为朊病毒病领域带来诊断和/或治疗方法。
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Cold Spring Harb Perspect Biol. 2016 Sep 1;8(9):a023515. doi: 10.1101/cshperspect.a023515.
2
Structure of the toxic core of α-synuclein from invisible crystals.来自不可见晶体的α-突触核蛋白毒性核心结构。
Nature. 2015 Sep 24;525(7570):486-90. doi: 10.1038/nature15368. Epub 2015 Sep 9.
3
Crystal Structures of Polymorphic Prion Protein β1 Peptides Reveal Variable Steric Zipper Conformations.多态性朊病毒蛋白β1肽的晶体结构揭示了可变的空间拉链构象。
Biochemistry. 2015 Jun 16;54(23):3640-8. doi: 10.1021/acs.biochem.5b00425. Epub 2015 Jun 3.
4
Parallel in-register intermolecular β-sheet architectures for prion-seeded prion protein (PrP) amyloids.朊病毒种子引发的朊蛋白（PrP）淀粉样蛋白的平行共配体分子间β-折叠结构
J Biol Chem. 2014 Aug 29;289(35):24129-42. doi: 10.1074/jbc.M114.578344. Epub 2014 Jul 15.
5
A proposed mechanism for the promotion of prion conversion involving a strictly conserved tyrosine residue in the β2-α2 loop of PrPC.一个涉及 PrPC 中β2-α2 环中严格保守的酪氨酸残基的朊病毒转化促进的拟议机制。
J Biol Chem. 2014 Apr 11;289(15):10660-10667. doi: 10.1074/jbc.M114.549030. Epub 2014 Mar 4.
6
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J Neurosci. 2014 Jan 15;34(3):1022-7. doi: 10.1523/JNEUROSCI.4636-13.2014.
7
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Elife. 2013 Jul 16;2:e00857. doi: 10.7554/eLife.00857.
9
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J Am Chem Soc. 2013 Jul 17;135(28):10202-5. doi: 10.1021/ja403001q. Epub 2013 Jul 2.
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