Department of Analytical Chemistry, College of Chemistry, Jilin University , Changchun 130012, China.
State Key Laboratory for Supramolecular Structure and Materials, College of Chemistry, Jilin University , Changchun 130012, China.
ACS Appl Mater Interfaces. 2017 Feb 15;9(6):5430-5436. doi: 10.1021/acsami.6b12455. Epub 2017 Feb 2.
In this paper, a facilely prepared electrochemiluminescence (ECL) biosensor was developed for Escherichia coli O157:H7 quantitative detection based on a polydopamine (PDA) surface imprinted polymer (SIP) and nitrogen-doped graphene quantum dots (N-GQDs). N-GQDs with a high quantum yield of 43.2% were synthesized. The uniform PDA SIP film for E. coli O157:H7 was established successfully with a facile route. The dopamine and target bacteria were electropolymerized directly on the electrode. After removal of the E. coli O157:H7 template, the established PDA SIP can selectively recognize E. coli O157:H7. Accordingly, E. coli O157:H7 polyclonal antibody (pAb) was labeled with N-GQDs. The bioconjugation of SIP-E. coli O157:H7/pAb-N-GQDs can generate intensive ECL irradiation with KSO. As a result, E. coli O157:H7 was detected with the ECL sensing system. Under optimal conditions, the linear relationships between the ECL intensity and E. coli O157:H7 concentration were obtained from 10 colony-forming units (CFU) mL to 10 CFU mL with a limit of detection of 8 CFU mL. The biosensor based on this SIP film was applied in water sample detection successfully. The N-GQD-based ECL analytical method for E. coli O157:H7 was reported for the first time. The sensing system had high selectivity to the target analyte, provided new opportunities for use, and increased the rate of disease diagnosis and treatment and the prevention of pathogens.
本文基于聚多巴胺（PDA）表面印迹聚合物（SIP）和氮掺杂石墨烯量子点（N-GQDs），开发了一种简便制备的用于定量检测大肠杆菌 O157:H7 的电化学发光（ECL）生物传感器。合成了量子产率为 43.2%的高荧光 N-GQDs。通过简便的方法成功建立了用于大肠杆菌 O157:H7 的均匀的 PDA SIP 膜。多巴胺和目标细菌直接在电极上电聚合。去除大肠杆菌 O157:H7 模板后，建立的 PDA SIP 可以选择性地识别大肠杆菌 O157:H7。相应地，大肠杆菌 O157:H7 多克隆抗体（pAb）用 N-GQDs 标记。SIP-大肠杆菌 O157:H7/pAb-N-GQDs 的生物缀合可以与 KSO 产生强烈的 ECL 辐射。因此，使用 ECL 传感系统检测大肠杆菌 O157:H7。在最佳条件下，从 10 个菌落形成单位 (CFU) mL 到 10 CFU mL，获得了 ECL 强度与大肠杆菌 O157:H7 浓度之间的线性关系，检测限为 8 CFU mL。该基于 SIP 膜的生物传感器成功应用于水样检测。首次报道了基于 N-GQD 的 ECL 分析方法用于检测大肠杆菌 O157:H7。该传感系统对目标分析物具有高选择性，为其应用提供了新的机会，并提高了疾病诊断和治疗以及病原体预防的速度。
