Haraguchi Naotsugu, Ohara Nobuyoshi, Koseki Jun, Takahashi Hidekazu, Nishimura Junichi, Hata Taishi, Mizushima Tsunekazu, Yamamoto Hirofumi, Ishii Hideshi, Doki Yuichiro, Mori Masaki
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
Department of Cancer Profiling Discovery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan.
Mol Clin Oncol. 2017 Jan;6(1):130-134. doi: 10.3892/mco.2016.1088. Epub 2016 Nov 21.
Members of the ADAMTS family contain propeptide, metalloproteinase and disintegrin domains and serve key roles for cancer cell proliferation, progression and metastasis. Although overexpression of ADAMTS5 has been reported in glioblastoma, and head and neck cancer, little has been demonstrated in colorectal cancer types. The present study aimed to clarify the significance of ADAMTS5 for clinicopathological factors and prognosis in colorectal cancer. The mRNA expression of ADAMTS5 was measured in 143 colorectal cancer specimens. ADAMTS5 expression was increased as the pathological stage increased. The expression of ADAMTS5 in stage III-IV colorectal cancer was significantly greater compared with that of stage 0-II colorectal cancer (P=0.0003). The median expression of ADAMTS5 was used to divide the ADAMTS5 higher expressing group and the ADAMTS5 lower expressing group to assess the correlation of ADAMTS5 expression with clinicopathological factors and prognosis. The proportions of lymphatic invasion and lymph node metastasis were significantly greater in the ADAMTS5 higher expressing group (P=0.0214 and P=0.0289 respectively). Overall survival and disease-free survival were assessed by the Kaplan-Meier curve with calculation of significance by the log-rank test; however, no significant difference was observed between the ADAMTS5 higher expressing group and the ADAMTS5 lower expressing group (P=0.7490 and P=0.9455, respectively). The present study confirmed high expression of ADAMTS5 as a potent marker for lymphatic invasion and lymphnode metastasis in colorectal cancer. To clarify the function of ADAMTS5 in colorectal cancer, further molecular investigations are required.
ADAMTS家族成员包含前肽、金属蛋白酶和解聚素结构域，在癌细胞增殖、进展和转移中发挥关键作用。尽管已有报道称ADAMTS5在胶质母细胞瘤、头颈癌中过表达，但在结直肠癌类型中鲜有相关研究。本研究旨在阐明ADAMTS5在结直肠癌临床病理因素及预后中的意义。对143例结直肠癌标本检测ADAMTS5的mRNA表达。ADAMTS5表达随病理分期增加而升高。III-IV期结直肠癌中ADAMTS5的表达显著高于0-II期结直肠癌（P=0.0003）。以ADAMTS5的中位表达量将其分为高表达组和低表达组，评估ADAMTS5表达与临床病理因素及预后的相关性。ADAMTS5高表达组的淋巴侵犯和淋巴结转移比例显著更高（分别为P=0.0214和P=0.0289）。采用Kaplan-Meier曲线评估总生存期和无病生存期，并通过对数秩检验计算显著性；然而，ADAMTS5高表达组和低表达组之间未观察到显著差异（分别为P=0.7490和P=0.9455）。本研究证实ADAMTS5高表达是结直肠癌淋巴侵犯和淋巴结转移的有效标志物。为阐明ADAMTS5在结直肠癌中的功能，需要进一步开展分子研究。
