Mahmoudvand Hossein, Saedi Dezaki Ebrahim, Sharififar Fariba, Ezatpour Behrouz, Jahanbakhsh Sareh, Fasihi Harandi Majid
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran ; Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences Kerman, Iran.
Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences Kerman, Iran.
Iran J Parasitol. 2014 Oct-Dec;9(4):503-10.
Cystic echinococcosis (CE), a zoonotic parasitic infection caused by the metacestode (larvae) stage of dog tapeworm Echinococcus granulosus and recognized as a major economic and public health concern in the world. This study aimed to investigate the in vitro scolicidal effect of methanolic extract of Berberis vulgaris L. roots and its main compound, berberine against protoscoleces of hydatid cysts.
For this purpose, protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the methanolic extract (0.25-2 mg/ml) and berberine (0.062- 0.5 mg/ml) were used for 5 to 30 min. Viability of protoscoleces was confirmed by eosin exclusive test.
In the present study, all of the various concentrations of the B. vulgaris methanolic extract (0.25, 0.5, 1 and 2 mg/ml) and berberine (0.062, 0.125, 0.25 and 0.5 mg/ml) revealed significant (P<0.05) scolicidal effects against protoscoleces of E. granulosus in a dose-dependent manner. Both berberine and methanolic extract exhibited 100% inhibition against protoscoleces of E. granulosus at the concentration of 2.0 and 0.5 mg/ml after 10 min incubation, respectively.
According to the results, both B. vulgaris methanolic extract and berberine alone demonstrated high scolicidal activities against protoscoleces of hydatid cysts in low concentration and short exposure time on in vitro model. However, in vivo efficacy of B. vulgaris and berberine also requires to be evaluated using an animal model with hydatid infection.
囊型包虫病（CE）是由犬绦虫细粒棘球绦虫的中绦期（幼虫）引起的一种人畜共患寄生虫感染，被认为是全球主要的经济和公共卫生问题。本研究旨在探讨小檗根甲醇提取物及其主要化合物黄连素对包虫囊肿原头节的体外杀头节作用。
为此，从患有包虫囊肿的绵羊肝脏中无菌吸出原头节。使用不同浓度的甲醇提取物（0.25 - 2mg/ml）和黄连素（0.062 - 0.5mg/ml）作用5至30分钟。通过伊红排斥试验确认原头节的活力。
在本研究中，不同浓度的小檗甲醇提取物（0.25、0.5、1和2mg/ml）和黄连素（0.062、0.125、0.25和0.5mg/ml）均以剂量依赖方式对细粒棘球绦虫原头节显示出显著（P<0.05）的杀头节作用。黄连素和甲醇提取物在孵育10分钟后，分别在浓度为2.0和0.5mg/ml时对细粒棘球绦虫原头节表现出100%的抑制作用。
根据结果，小檗甲醇提取物和黄连素单独使用在体外模型中低浓度和短暴露时间下均对包虫囊肿原头节显示出高杀头节活性。然而，小檗和黄连素的体内疗效也需要使用包虫感染动物模型进行评估。
