Virginio Veridiana Gomes, Bandeira Natalia Costantin, Leal Fernanda Munhoz Dos Anjos, Lancellotti Marcelo, Zaha Arnaldo, Ferreira Henrique Bunselmeyer
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Biologia Celular e Molecular, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
Heliyon. 2017 Jan 6;3(1):e00225. doi: 10.1016/j.heliyon.2016.e00225. eCollection 2017 Jan.
The adjuvant potential of two mesoporous silica nanoparticles (MSNs), SBa-15 and SBa-16, was assessed in combination with a recombinant HSP70 surface polypeptide domain from , the etiological agent of porcine enzootic pneumonia (PEP). The recombinant antigen (HSP70), previously shown as immunogenic in formulation with classic adjuvants, was used to immunize BALB/c mice in combination with SBa-15 or SBa-16 MSNs, and the effects obtained with these formulations were compared to those obtained with alum, the adjuvant traditionally used in anti-PEP bacterins. The HSP70 + SBa-15 vaccine elicited a strong humoral immune response, with high serum total IgG levels, comparable to those obtained using HSP70 + alum. The HSP70 + SBa-16 vaccine elicited a moderate humoral immune response, with lower levels of total IgG. The cellular immune response was assessed by the detection of IFN-γ, IL-4 and IL-10 in splenocyte culture supernatants. The HSP70 + SBa-15 vaccine increased IFN-γ, IL-4 and IL-10 levels, while no stimulation was detected with the HSP70 + SBa-16 vaccine. The HSP70 + SBa-15 vaccine induced a mixed Th1/Th2-type response, with an additional IL-10 mediated anti-inflammatory effect, both of relevance for an anti-PEP vaccine. Alum adjuvant controls stimulated an unspecific cellular immune response, with similar levels of cytokines detected in mice immunized either with HSP70 + alum or with the adjuvant alone. The better humoral and cellular immune responses elicited in mice indicated that SBa-15 has adjuvant potential, and can be considered as an alternative to the use of alum in veterinary vaccines. The use of SBa-15 with HSP70 is also promising as a potential anti-PEP subunit vaccine formulation.
评估了两种介孔二氧化硅纳米颗粒（MSN），即SBa - 15和SBa - 16与来自猪地方流行性肺炎（PEP）病原体的重组HSP70表面多肽结构域联合使用时的佐剂潜力。先前已证明重组抗原（HSP70）与经典佐剂联合使用具有免疫原性，将其与SBa - 15或SBa - 16 MSN联合用于免疫BALB/c小鼠，并将这些制剂产生的效果与传统用于抗PEP菌苗的佐剂明矾所产生的效果进行比较。HSP70 + SBa - 15疫苗引发了强烈的体液免疫反应，血清总IgG水平较高，与使用HSP70 +明矾时相当。HSP70 + SBa - 16疫苗引发了中等程度的体液免疫反应，总IgG水平较低。通过检测脾细胞培养上清液中的IFN - γ、IL - 4和IL - 10来评估细胞免疫反应。HSP70 + SBa - 15疫苗提高了IFN - γ、IL - 4和IL - 10水平，而HSP70 + SBa - 16疫苗未检测到刺激作用。HSP70 + SBa - 15疫苗诱导了混合的Th1/Th2型反应，并具有额外的IL - 10介导的抗炎作用，这两者对于抗PEP疫苗都具有相关性。明矾佐剂对照组刺激了非特异性细胞免疫反应，在用HSP70 +明矾或单独使用佐剂免疫的小鼠中检测到的细胞因子水平相似。在小鼠中引发的更好的体液和细胞免疫反应表明SBa - 15具有佐剂潜力，可被视为兽用疫苗中使用明矾的替代品。将SBa - 15与HSP70联合使用作为潜在的抗PEP亚单位疫苗制剂也很有前景。
