Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.
Center for Advanced Technologies for the Preservation of Biological Systems (ATP-Bio), University of Minnesota, Minneapolis, MN, 55455, USA.
Adv Sci (Weinh). 2024 Aug;11(32):e2404617. doi: 10.1002/advs.202404617. Epub 2024 Jun 21.
Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism-integrated devices. This requires continuously assessing the locations of randomly distributed organisms to collect and transfer them to target spaces without harm. Here, an aspiration-assisted adaptive printing system is developed that tracks, harvests, and relocates living and moving organisms on target spaces via a pick-and-place mechanism that continuously adapts to updated visual and spatial information about the organisms and target spaces. These adaptive printing strategies successfully positioned a single static organism, multiple organisms in droplets, and a single moving organism on target spaces. Their capabilities are exemplified by printing vitrification-ready organisms in cryoprotectant droplets, sorting live organisms from dead ones, positioning organisms on curved surfaces, organizing organism-powered displays, and integrating organisms with materials and devices in customizable shapes. These printing strategies can ultimately lead to autonomous biomanufacturing methods to evaluate and assemble organisms for a variety of single and multi-organism-based applications.
设计一种能够确定位置的生物体的方法可以影响生物成像、控制论、冷冻保存和与生物体集成的设备等各个领域。这需要不断评估随机分布的生物体的位置，以便收集并将它们转移到目标空间而不会造成伤害。在这里，开发了一种吸气辅助自适应打印系统，该系统通过一种拾放机制来跟踪、收获和重新定位目标空间上的活体和移动生物体，该机制不断适应生物体和目标空间的更新视觉和空间信息。这些自适应打印策略成功地将单个静态生物体、多个液滴中的生物体和单个移动生物体定位在目标空间上。通过将可进行玻璃化处理的生物体打印在防冻剂液滴中、从死亡生物体中分拣活生物体、在曲面上定位生物体、组织生物体驱动的显示器以及以可定制形状将生物体与材料和设备集成，展示了它们的功能。这些打印策略最终可以实现自主生物制造方法，以评估和组装各种基于单个和多个生物体的应用。
