Barbieri Antonio, Quagliariello Vincenzo, Del Vecchio Vitale, Falco Michela, Luciano Antonio, Amruthraj Nagoth Joseph, Nasti Guglielmo, Ottaiano Alessandro, Berretta Massimiliano, Iaffaioli Rosario Vincenzo, Arra Claudio
Animal Facility Unit, Department of Research, National Cancer Institute "G. Pascale", Via M. Semmola, 80131 Naples, Italy.
Department of Abdominal Oncology, National Cancer Institute "G. Pascale", Via M. Semmola, 80131 Naples, Italy.
Nutrients. 2017 Feb 28;9(3):210. doi: 10.3390/nu9030210.
Among the most important traditional medicinal fungi, has been used as a therapeutic agent for the treatment of numerous diseases, including cancer, in Oriental countries. The aim of this study is to investigate the anti-inflammatory, anticancer and anti-metastatic activities of extracts in melanoma and triple-negative breast cancer cells. extracts were prepared by using common organic solvents; MDA-MB 231 and B16-F10 cell lines were adopted as cellular models for triple-negative breast cancer and melanoma and characterized for cell viability, wound-healing assay and measurement of cytokines secreted by cancer cells under pro-inflammatory conditions (incubation with lipopolysaccharide, LPS) and pretreatment with extract at different concentrations. Our study demonstrates, for the first time, how extracts can significantly inhibit the release of IL-8, IL-6, MMP-2 and MMP-9 in cancer cells under pro-inflammatory condition. Interestingly, extracts significantly also decrease the viability of both cancer cells in a time- and concentration-dependent manner, with abilities to reduce cell migration over time, which is correlated with a lower release of matrix metalloproteases. Taken together, these results indicate the possible use of extract for the therapeutic management of melanoma and human triple-negative breast cancer.
在最重要的传统药用真菌中，在东方国家已被用作治疗包括癌症在内的多种疾病的治疗剂。本研究的目的是研究提取物在黑色素瘤和三阴性乳腺癌细胞中的抗炎、抗癌和抗转移活性。提取物通过使用常见有机溶剂制备；采用MDA-MB 231和B16-F10细胞系作为三阴性乳腺癌和黑色素瘤的细胞模型，并对细胞活力、伤口愈合试验以及在促炎条件下（用脂多糖LPS孵育）癌细胞分泌的细胞因子以及用不同浓度的提取物预处理进行表征。我们的研究首次证明了提取物如何在促炎条件下显著抑制癌细胞中IL-8、IL-6、MMP-2和MMP-9的释放。有趣的是，提取物还以时间和浓度依赖性方式显著降低两种癌细胞的活力，随着时间的推移具有降低细胞迁移的能力，这与基质金属蛋白酶的较低释放相关。综上所述，这些结果表明提取物可能用于黑色素瘤和人类三阴性乳腺癌的治疗管理。
