口腔扁平苔藓组织中唾液支原体的免疫组织化学检测。

Immunohistochemical detection of Mycoplasma salivarium in oral lichen planus tissue.

机构信息

Division of Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Reconstructive Surgery, School of Dentistry, Iwate Medical University, Morioka, Iwate, Japan.

Division of Anatomical and Cellular Pathology, Department of Pathology, Iwate Medical University, Morioka, Iwate, Japan.

出版信息

J Oral Pathol Med. 2017 Sep;46(8):649-656. doi: 10.1111/jop.12568. Epub 2017 Apr 6.

DOI:10.1111/jop.12568

PMID:28295632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5600092/

Abstract

BACKGROUND

Oral lichen planus (OLP) is a T-cell-mediated inflammatory disease; however, its exact etiology is unknown. Hyperkeratosis is often observed in OLP lesions. Previous studies have revealed the localization of Mycoplasma salivarium in the epithelial cells of oral leukoplakia with hyperkeratosis. Herein, we investigated the presence of M. salivarium in OLP tissue by immunohistochemistry to determine the causative factor of OLP.

METHODS

Forty-one formalin-fixed, paraffin-embedded samples obtained from 31 patients with OLP were examined. Ten samples of normal-appearing oral mucosa were used as controls. Immunohistochemistry (IHC) was performed using anti-M. salivarium monoclonal antibodies.

RESULTS AND CONCLUSIONS

Mycoplasma salivarium was detected in the epithelium and lymphocyte infiltrate area in 24 of 41 OLP samples (58.5%). The bacteria were intracellularly localized in epithelial cells, while it was unclear whether they were also localized in lymphocyte cells or in the extracellular spaces among the lymphocytes in the subepithelial lymphocyte infiltrate area. Little or no staining was observed in the epithelium in the normal-appearing mucosa samples. Sawtooth rete ridge formation was observed in 21 OLP samples (51.2%), and a significant positive correlation between sawtooth rete ridge formation and IHC positivity was demonstrated. However, the role of M. salivarium in the epithelium and lamina propria of OLP tissue remains unknown.

摘要

背景

口腔扁平苔藓（OLP）是一种 T 细胞介导的炎症性疾病；然而，其确切病因尚不清楚。角化过度通常在 OLP 病变中观察到。先前的研究已经揭示了唾液支原体在伴有角化过度的口腔白斑病上皮细胞中的定位。在此，我们通过免疫组织化学法研究 M. salivarium 在 OLP 组织中的存在，以确定 OLP 的致病因素。

方法

对 31 例 OLP 患者的 41 例福尔马林固定、石蜡包埋样本进行了检查。10 例正常口腔黏膜样本作为对照。使用抗 M. salivarium 单克隆抗体进行免疫组织化学（IHC）。

结果与结论

在 41 例 OLP 样本中的 24 例（58.5%）中检测到 M. salivarium 存在于上皮和淋巴细胞浸润区。细菌在上皮细胞内定位于上皮细胞内，而不清楚其是否也位于淋巴细胞内或在下皮内淋巴细胞浸润区的淋巴细胞之间的细胞外空间内。在正常外观的黏膜样本的上皮中观察到很少或没有染色。在 21 例 OLP 样本中观察到锯齿状网嵴形成（51.2%），并且证明锯齿状网嵴形成与 IHC 阳性之间存在显著正相关。然而，M. salivarium 在 OLP 组织的上皮和固有层中的作用尚不清楚。