Wang Xuewei, Zhao Zhibai, Tang Nan, Zhao Yuping, Xu Juanyong, Li Liuyang, Qian Ling, Zhang Junfeng, Fan Yuan
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, China.
Department of Oral Medicine, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, China.
Front Microbiol. 2020 May 5;11:629. doi: 10.3389/fmicb.2020.00629. eCollection 2020.
The specific etiology and pathogenesis of oral lichen planus (OLP) remain elusive, and microbial dysbiosis may play an important role in OLP. We evaluated the saliva and tissue bacterial community of patients with OLP and identified the colonization of bacteria in OLP tissues. The saliva ( = 60) and tissue ( = 24) samples from OLP patients and the healthy controls were characterized by 16S rDNA gene sequencing and the bacterial signals in OLP tissues were detected by fluorescence hybridization (FISH) targeting the bacterial 16S rDNA gene. Results indicate that the OLP tissue microbiome was different from the microbiota of OLP saliva. Compared with the healthy controls, and were higher in OLP saliva, while - and were higher in OLP tissues, whereas seven taxa, including Carnobacteriaceae, Flavobacteriaceae, and , were enriched in both saliva and tissues of OLP patients. Furthermore, FISH found that the average optical density (AOD) of bacteria in the lamina propria of OLP tissues was higher than that of the healthy controls, and the AOD of bacteria in OLP epithelium and lamina propria was positively correlated. These data provide a different perspective for future investigation on the OLP microbiome.
口腔扁平苔藓（OLP）的具体病因和发病机制仍不清楚，微生物群落失调可能在OLP中起重要作用。我们评估了OLP患者的唾液和组织细菌群落，并确定了OLP组织中细菌的定植情况。通过16S rDNA基因测序对OLP患者（n = 60）和健康对照者的唾液（n = 60）及组织（n = 24）样本进行特征分析，并通过靶向细菌16S rDNA基因的荧光原位杂交（FISH）检测OLP组织中的细菌信号。结果表明，OLP组织微生物群与OLP唾液微生物群不同。与健康对照相比，OLP唾液中的链球菌属和韦荣球菌属较高，而OLP组织中的卟啉单胞菌属和普氏菌属较高，而包括肉杆菌科、黄杆菌科等在内的7个分类群在OLP患者的唾液和组织中均富集。此外，FISH发现OLP组织固有层中细菌的平均光密度（AOD）高于健康对照，且OLP上皮和固有层中细菌的AOD呈正相关。这些数据为未来对OLP微生物群的研究提供了不同的视角。
