Luebke Jan, Anton Alexandra, Bach Michael
Eye Center, Medical Center, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany.
Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Doc Ophthalmol. 2017 Jun;134(3):157-165. doi: 10.1007/s10633-017-9582-1. Epub 2017 Mar 16.
To explore test-retest reliability of standard scotopic full-field ERG measurements in New Zealand White rabbits. The ERG is widely used for testing of retinal integrity after any ocular treatment. We here present detailed stimulus-response dependencies for single healthy and untreated animals, concentrating on test-retest reproducibility.
Five New Zealand White rabbits (aged 8-10 weeks, weight about 2.0-2.5 kg) underwent binocular ERG measurements after intramuscular anesthesia and pharmaceutical pupillary dilatation at a baseline day and 10 days later. Eleven scotopic flash strengths (0.0001-10 cd s/m) were presented. Variability was quantified via the 95% limits of agreement (LOA).
The a-waves displayed the typical monotonic sigmoid amplitude increase with flash strength, and the b-waves peaked at 0.01 cd s/m, followed by a marked dip at 0.1-0.3 cd s/m. LOA of both waves went through a maximum in the dip region. LOA divided by mean amplitudes (relative LOA) was fairly flat over flash strength, around 20% beyond the dip. Intraindividual interocular variability was markedly lower, around 10%.
Scotopic ERG responses in rabbits display a region of high variability at 0.1-0.3 cd s/m; beyond that region the amplitude-LOA is 20%, the interocular LOA being half that value. The use of intraindividual control eyes for testing any toxicity of ocular agents thus appears markedly more sensitive. As a rule of thumb, we found the relative 95% LOA as 33% between individuals, 20% across sessions and 10% between eyes.
探讨新西兰白兔标准暗视全视野视网膜电图（ERG）测量的重测信度。ERG被广泛用于任何眼部治疗后视网膜完整性的检测。我们在此展示了单个健康未治疗动物详细的刺激-反应依赖性，重点关注重测的可重复性。
五只新西兰白兔（8 - 10周龄，体重约2.0 - 2.5千克）在基线日及10天后，经肌肉麻醉和药物性瞳孔散大后进行双眼ERG测量。呈现了11种暗视闪光强度（0.0001 - 10 cd·s/m²）。通过95%一致性界限（LOA）对变异性进行量化。
a波随闪光强度呈现典型的单调S形振幅增加，b波在0.01 cd·s/m²时达到峰值，随后在0.1 - 0.3 cd·s/m²处出现明显下降。两个波的LOA在下降区域达到最大值。LOA除以平均振幅（相对LOA）在闪光强度范围内相当平稳，在下降区域之外约为20%。个体内眼间变异性明显更低，约为10%。
兔的暗视ERG反应在0.1 - 0.3 cd·s/m²区域显示出高变异性；在该区域之外，振幅-LOA为20%，眼间LOA为该值的一半。因此，使用个体内对照眼来检测眼用药物的任何毒性似乎明显更敏感。根据经验，我们发现个体间相对95% LOA为33%，不同测量时段间为20%，眼间为10%。
