Darko Eva, Gierczik Krisztián, Hudák Orsolya, Forgó Péter, Pál Magda, Türkösi Edina, Kovács Viktória, Dulai Sándor, Majláth Imre, Molnár István, Janda Tibor, Molnár-Láng Márta
Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
Department of Plant Molecular Biology, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.
PLoS One. 2017 Mar 22;12(3):e0174170. doi: 10.1371/journal.pone.0174170. eCollection 2017.
Salinity-induced osmotic, ionic and oxidative stress responses were investigated on Asakaze/Manas wheat/barley addition lines 7H, 7HL and 7HS, together with their barley (salt-tolerant) and wheat (relatively salt-sensitive) parents. Growth, photosynthetic activity, chlorophyll degradation, proline, glycine betaine accumulation, sugar metabolism, Na+ and K+ uptake and transport processes and the role of polyamines and antioxidants were studied in young plants grown in hydroponic culture with or without salt treatment. Changes in plant growth and photosynthetic activity of plants demonstrated that the salt tolerance of the addition lines 7H and 7HL was similar to that of barley parent cv. Manas, while the sensitivity of the addition line 7HS was similar to that of the wheat parent cv. Asakaze. The Na accumulation in the roots and shoots did not differ between the addition lines and wheat parent. The activation of various genes related to Na uptake and transport was not correlated with the salt tolerance of the genotypes. These results indicated that the direct regulation of Na transport processes is not the main reason for the salt tolerance of these genotypes. Salt treatment induced a complex metabolic rearrangement in both the roots and shoots of all the genotypes. Elevated proline accumulation in the roots and enhanced sugar metabolism in the shoots were found to be important for salt tolerance in the 7H and 7HL addition lines and in barley cv. Manas. In wheat cv. Asakaze and the 7HS addition line the polyamine metabolism was activated. It seems that osmotic adjustment is a more important process in the improvement of salt tolerance in 7H addition lines than the direct regulation of Na transport processes or antioxidant defence.
研究了盐胁迫诱导的渗透、离子和氧化应激反应，对象为浅间风/马纳斯小麦/大麦附加系7H、7HL和7HS，以及它们的大麦（耐盐）和小麦（相对盐敏感）亲本。在水培条件下生长的幼苗中，研究了有无盐处理时的生长、光合活性、叶绿素降解、脯氨酸、甘氨酸甜菜碱积累、糖代谢、Na⁺和K⁺吸收与运输过程以及多胺和抗氧化剂的作用。植株生长和光合活性的变化表明，附加系7H和7HL的耐盐性与大麦亲本品种马纳斯相似，而附加系7HS的敏感性与小麦亲本品种浅间风相似。附加系和小麦亲本在根和地上部的Na积累没有差异。与Na吸收和运输相关的各种基因的激活与基因型的耐盐性不相关。这些结果表明，Na运输过程的直接调控不是这些基因型耐盐性的主要原因。盐处理在所有基因型的根和地上部都诱导了复杂的代谢重排。发现根中脯氨酸积累增加和地上部糖代谢增强对7H和7HL附加系以及大麦品种马纳斯的耐盐性很重要。在小麦品种浅间风和7HS附加系中，多胺代谢被激活。似乎渗透调节在提高7H附加系的耐盐性方面比直接调控Na运输过程或抗氧化防御更为重要。
