Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India.
Centre of Excellence in Materials Science (Nanomaterials), Department of Applied Physics, Z. H. College of Engineering and Technology, Aligarh Muslim University, Aligarh, India.
Sci Rep. 2017 Mar 27;7:45154. doi: 10.1038/srep45154.
Stenotrophomonas sp. is emerging as a popular microbe of global concern with various potential ecological roles. Biosynthesis of gold and silver nanoparticles (AgNPs) using this bacterial strain has shown promising applications in life sciences. However, there is no report on efficient agricultural applications of biosynthesized AgNPs using Stenotrophomonas sp. In this regard, successful biosynthesis of AgNPs using Stenotrophomonas sp. BHU-S7 (MTCC 5978) was monitored by Uv-visible spectrum showing surface plasmon resonance (SPR) peak at 440 nm. The biosynthesized AgNPs were spherical with an average mean size of ~12 nm. The antifungal efficacy of biosynthesized AgNPs against foliar and soil-borne phytopathogens was observed. The inhibitory impact of AgNPs (2, 4, 10 μg/ml) on conidial germination was recorded under in vitro conditions. Interestingly, sclerotia of Sclerotium rolfsii exposed to AgNPs failed to germinate on PDA medium and in soil system. Moreover, AgNPs treatment successfully managed collar rot of chickpea caused by S. rolfsii under greenhouse conditions. The reduced sclerotia germination, phenolic acids induction, altered lignification and HO production was observed to be the probable mechanisms providing protection to chickpea against S. rolfsii. Our data revealed that AgNPs treated plants are better equipped to cope with pathogen challenge pointing towards their robust applications in plant disease management.
茎单胞菌属正在成为一种受到全球关注的流行微生物，具有各种潜在的生态作用。使用该细菌菌株合成金和银纳米粒子（AgNPs）已经显示出在生命科学中具有广阔的应用前景。然而，目前还没有关于使用茎单胞菌属合成的 AgNPs 在农业上的高效应用的报道。在这方面，成功地使用 Stenotrophomonas sp. BHU-S7（MTCC 5978）合成了 AgNPs，通过 Uv-可见光谱监测到表面等离子体共振（SPR）峰在 440nm 处。合成的 AgNPs 呈球形，平均粒径约为 12nm。观察了合成的 AgNPs 对叶部和土壤传播植物病原菌的抗真菌功效。在体外条件下记录了 AgNPs（2、4、10μg/ml）对孢子萌发的抑制作用。有趣的是，暴露于 AgNPs 的 Sclerotium rolfsii 菌核在 PDA 培养基和土壤系统中未能萌发。此外，AgNPs 处理成功地管理了温室条件下由 S. rolfsii 引起的鹰嘴豆根腐病。观察到菌核萌发减少、酚酸诱导、木质化改变和 HO 产生，这可能是为鹰嘴豆提供对 S. rolfsii 保护的机制。我们的数据表明，AgNPs 处理的植物更好地应对病原体的挑战，这表明它们在植物病害管理中有很强的应用潜力。
