NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton 3800, Victoria, Australia.
NanoScience and Sensor Technology Research Group, School of Chemistry, Monash University, Clayton 3800, Victoria, Australia.
Food Chem. 2017 Aug 15;229:127-135. doi: 10.1016/j.foodchem.2017.01.138. Epub 2017 Jan 31.
A novel polypyrrole (PPy)-based bilayer amperometric glucose biosensor integrated with a permselective layer has been developed for detection of glucose in the presence of interferences. It comprises of a PPy-GOx film grown, in the absence of electrolyte, as an inner layer, and a permselective PPy-Cl film as an outer layer. The PPy-GOx/PPy-Cl bilayer biosensor was effective in rejecting 98% of ascorbic acid and 100% of glycine, glutamic acid and uric acid. With an outer layer thickness of 6.6nm, the bilayer biosensor gave nearly identical glucose response to that of a single layer PPy-GOx biosensor. The biosensor also exhibited good reproducibility (1.9% rsd, n=10), high stability (more than 2months), wide linear range (0.5-24mM), low K (8.4mM), high I (77.2μAcm), low detection limit (26.9μM) and good sensitivity (3.5μAcmmM). The bilayer biosensor was successfully employed for glucose determination in various fruit juices.
一种新型的聚吡咯（PPy）双层安培葡萄糖生物传感器与选择性渗透膜结合，用于在存在干扰的情况下检测葡萄糖。它由一个在没有电解质的情况下生长的 PPy-GOx 薄膜作为内层，和一个作为外层的选择性渗透 PPy-Cl 薄膜组成。PPy-GOx/PPy-Cl 双层生物传感器能够有效地排除 98%的抗坏血酸和 100%的甘氨酸、谷氨酸和尿酸。在外层厚度为 6.6nm 时，双层生物传感器对葡萄糖的响应与单层 PPy-GOx 生物传感器几乎相同。该生物传感器还表现出良好的重现性（1.9%的 rsd，n=10）、高稳定性（超过 2 个月）、宽线性范围（0.5-24mM）、低 K 值（8.4mM）、高 I 值（77.2μAcm）、低检测限（26.9μM）和良好的灵敏度（3.5μAcmmM）。双层生物传感器成功地用于各种果汁中的葡萄糖测定。
