McMullin David R, Green Blake D, Prince Natasha C, Tanney Joey B, Miller J David
Ottawa Carleton Institute of Chemistry, Carleton University , 1125 Colonel By Drive, Ottawa, Ontario, Canada , K1S 5B6.
J Nat Prod. 2017 May 26;80(5):1475-1483. doi: 10.1021/acs.jnatprod.6b01157. Epub 2017 Apr 11.
Endophytes of healthy needles were collected from Picea rubens (red spruce) and P. mariana (black spruce) in a survey of southeastern New Brunswick, Canada. Four endophyte strains were selected for further investigation based on the production of biologically active extracts from culture filtrates during screening as well as phylogenetic relationship to species known to produce natural products or taxonomic novelty. A novel endophyte within the family Rhytismataceae produced two new dihydropyrones (1 and 2) as major metabolites together with phthalides (3 and 4), isocoumarins (5 and 6), and tyrosol (7). Lachnum cf. pygmaeum synthesized a new chlorinated para-quinone, chloromycorrhizinone A (8), and the nematicidal compounds (1'Z)-dechloromycorrhizin A (9), mycorrhizin A (10), and chloromycorrhizin A (11). A new isocoumarin (12) and four related structures (13-16) were isolated from an undescribed taxon in the Mycosphaerellaceae. The known antifungal metabolites cryptosporiopsin (17), 5-hydroxycryptosporiopsin (18), (+)-cryptosporiopsinol (19), and mellein (20) were produced by Pezicula sporulosa. Phylogenetically diverse conifer endophytes from the Acadian forest continue to be a productive source of new biologically active natural products.
在加拿大新不伦瑞克省东南部的一项调查中，从红云杉（Picea rubens）和黑云杉（P. mariana）的健康针叶中收集了内生菌。在筛选过程中，基于培养滤液中生物活性提取物的产生以及与已知产生天然产物或分类学新特性的物种的系统发育关系，选择了4种内生菌菌株进行进一步研究。座囊菌科中的一种新型内生菌产生了两种新的二氢吡喃酮（1和2）作为主要代谢产物，同时还产生了苯酞（3和4）、异香豆素（5和6）以及酪醇（7）。近似矮小粒毛盘菌（Lachnum cf. pygmaeum）合成了一种新的氯化对苯醌，即氯菌根菌素A（8），以及杀线虫化合物（1'Z）-脱氯菌根菌素A（9）、菌根菌素A（10）和氯菌根菌素A（11）。从球腔菌科中一个未描述的分类单元中分离出一种新的异香豆素（12）和四种相关结构（13 - 16）。已知的抗真菌代谢产物隐孢菌素（17）、5-羟基隐孢菌素（18）、（+）-隐孢菌素醇（19）和蜜环菌酸（20）由孢子状皮盘菌（Pezicula sporulosa）产生。来自阿卡迪亚森林的系统发育多样的针叶树内生菌仍然是新的生物活性天然产物的丰富来源。
