Ozturk Barcin, Kurtoglu Tunay, Durmaz Selim, Kozaci Leyla Didem, Abacigil Filiz, Ertugrul Bulent, Erel Ozcan
Assistant Professor, Department of Infectious Diseases and Clinical Microbiology, University of Adnan Menderes, School of Medicine, Aydin, Turkey. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation and writing.
Associate Professor, Department of Cardiovascular Surgery, University of Adnan Menderes, School of Medicine, Aydin, Turkey. Conception and design of the study, acquisition of data, technical procedures, manuscript preparation and writing.
Acta Cir Bras. 2017 Mar;32(3):219-228. doi: 10.1590/S0102-865020170030000006.
: To investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated.
: Forty adult Wistar rats were divided into Sham, Control, Vancomycin, Ozone, Vancomycin+Ozone groups. Grafts were inoculated with Methicillin-resistant Staphylococcus aureus (MRSA) strain and implanted subcutaneously. Rats were treated intraperitoneally with ozone and /or intramuscularly with vancomycin for 10 days. Grafts were evaluated by quantitative bacterial cultures. Blood samples were harvested for determination of thiol-disulphide and cytokine profiles.
: There was no significant difference in bacterial counts between Control and Ozone Groups. In the Ozone Group median colony count was significantly higher than the Vancomycin and Vancomycin+Ozone Groups. Total thiol and disulphide levels increased and disulphide/native thiol and disulphide/total thiol ratios decreased in Ozone Group significantly. Albumin levels decreased significantly in Vancomycin and Vancomycin+Ozone Groups compared to the Sham Group. IL-1 and TNF-alpha levels significantly increased in infected rats. Decreased levels of VEGF due to infection reversed by ozone therapy in control and vancomycin groups.
: We didn't observe any benefit of the agent on MRSA elimination in our model. Likewise, effects of ozone on thiol-disulphide homeostasis and inflammatory cytokines were contradictory.
研究辅助性臭氧给药在先前未被研究过的实验性大鼠血管移植物感染模型中的微生物学、炎症和氧化作用。
将40只成年Wistar大鼠分为假手术组、对照组、万古霉素组、臭氧组、万古霉素+臭氧组。将移植物接种耐甲氧西林金黄色葡萄球菌（MRSA）菌株并皮下植入。大鼠腹腔注射臭氧和/或肌肉注射万古霉素，持续10天。通过定量细菌培养评估移植物。采集血样以测定硫醇-二硫化物和细胞因子谱。
对照组和臭氧组之间的细菌计数无显著差异。臭氧组的菌落计数中位数显著高于万古霉素组和万古霉素+臭氧组。臭氧组的总硫醇和二硫化物水平显著升高，二硫化物/天然硫醇和二硫化物/总硫醇比值显著降低。与假手术组相比，万古霉素组和万古霉素+臭氧组的白蛋白水平显著降低。感染大鼠的白细胞介素-1和肿瘤坏死因子-α水平显著升高。在对照组和万古霉素组中，感染导致的血管内皮生长因子水平降低通过臭氧治疗得以逆转。
在我们的模型中，未观察到该药物对消除MRSA有任何益处。同样，臭氧对硫醇-二硫化物稳态和炎症细胞因子的影响相互矛盾。
