Prywes R, Dutta A, Cromlish J A, Roeder R G
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1988 Oct;85(19):7206-10. doi: 10.1073/pnas.85.19.7206.
Serum and growth factor regulation of c-FOS protooncogene transcription is mediated by the serum response element. A factor, serum response factor, binding to this element has already been identified. We demonstrate that serum response factor is phosphorylated in vivo on serine residues and that phosphatase treatment of this factor in vitro abolishes its DNA-binding activity. These results show phosphorylation of serum response factor to be required for its DNA-binding activity. The importance of serum response factor phosphorylation for the regulation of c-FOS expression is discussed.
c-FOS原癌基因转录的血清和生长因子调节是由血清反应元件介导的。一种与该元件结合的因子，即血清反应因子，已经被鉴定出来。我们证明血清反应因子在体内丝氨酸残基上发生磷酸化，并且在体外对该因子进行磷酸酶处理会消除其DNA结合活性。这些结果表明血清反应因子的磷酸化是其DNA结合活性所必需的。文中讨论了血清反应因子磷酸化对c-FOS表达调节的重要性。
