日本血吸虫感染诱导的 C57BL/6 鼠肝中白细胞介素-9 的特征。
Characteristics of IL-9 induced by Schistosoma japonicum infection in C57BL/6 mouse liver.
机构信息
Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, Sino-French Hoffmann Institute, The Second Affiliated Hospital, Guangzhou Medical University, 511436, Guangzhou, China.
Functional Experiment Centre, Guangzhou Medical University, 511436, Guangzhou, China.
出版信息
Sci Rep. 2017 May 24;7(1):2343. doi: 10.1038/s41598-017-02422-8.
Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qRT-PCR, and ELISA results demonstrated that the expression of IL-9 significantly increased after infection (P < 0.01). FACS results indicated that the peak of IL-9 Th9 cells in the liver mononuclear cells appeared at the early phase of infection (week 5), except that Th9 cells, CD8 Tc cells, NKT and γδT cells could secrete IL-9 in this model. Although IL-9 neutralization has a limited effect on liver granulomatous inflammation, it could decrease the level of fibrosis-associated factor, PC-III, in the serum of infected mice (P < 0.05). Taken together, our results indicated that IL-9 was an important type of cytokine involved in the progression of S. japonicum infection-induced hepatic damage.
肝肉芽肿性炎症和纤维化是日本血吸虫（S. japonicum）感染后观察到的主要病理变化。本研究探讨了白细胞介素-9（IL-9）在日本血吸虫感染 C57BL/6 小鼠肝脏中的特征。免疫荧光、qRT-PCR 和 ELISA 结果表明，感染后 IL-9 的表达显著增加（P<0.01）。FACS 结果表明，在感染早期（第 5 周），肝单核细胞中 IL-9 Th9 细胞的峰值出现，除了 Th9 细胞、CD8 Tc 细胞、NKT 和 γδT 细胞外，该模型中还可以分泌 IL-9。虽然 IL-9 中和对肝肉芽肿性炎症的影响有限，但它可以降低感染小鼠血清中纤维化相关因子 PC-III 的水平（P<0.05）。综上所述，我们的结果表明，IL-9 是一种参与日本血吸虫感染诱导肝损伤进展的重要细胞因子。
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