用生物素化DNA探针检测单纯疱疹病毒

Detection of herpes simplex virus by biotinylated DNA probes.

作者信息

Qadri S M, Qadri S G, Khan G Y, McGarry T J, Al-Ahdal M N

机构信息

Department of Pathology and Laboratory Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.

出版信息

Diagn Microbiol Infect Dis. 1988 Nov;11(3):145-9. doi: 10.1016/0732-8893(88)90016-8.

DOI:10.1016/0732-8893(88)90016-8

PMID:2854512

Abstract

Clinical specimens from 159 patients suspected with herpes simplex virus (HSV) were examined by monoclonal antibody immunofluorescence (IF) and by a commercial biotinylated DNA probe kit following cell culture isolation. Herpes simplex virus was isolated from 57 samples. All cultures were positive by IF when the cytopathic effect (CPE) was less than 1+ but only 49 (86%) yielded positive reaction with the DNA probe when CPE was at least 1+. A total of 54 clinical specimens was also examined directly by immunoperoxidase histopathology (IHP), IF, and DNA hybridization. Of these, 16 were positive by IHP, 15 by IF, and only five by DNA probe. The DNA probe kit was found to be reasonably sensitive only after cell culture isolation of HSV. Compared to the IF procedure, the DNA probe kit was found to be costly, labor intensive, and time consuming.

摘要

对159例疑似单纯疱疹病毒（HSV）感染患者的临床标本，在进行细胞培养分离后，采用单克隆抗体免疫荧光法（IF）和市售生物素化DNA探针试剂盒进行检测。从57份样本中分离出单纯疱疹病毒。当细胞病变效应（CPE）小于1+时，所有培养物经IF检测均为阳性，但当CPE至少为1+时，只有49份（86%）与DNA探针产生阳性反应。另外，对54份临床标本还直接采用免疫过氧化物酶组织病理学（IHP）、IF和DNA杂交法进行检测。其中，IHP检测阳性16份，IF检测阳性15份，DNA探针检测阳性仅5份。发现DNA探针试剂盒仅在HSV经细胞培养分离后才具有合理的敏感性。与IF方法相比，DNA探针试剂盒成本高、 labor intensive、耗时。（注：“labor intensive”直译为“劳动密集型”，结合语境这里可意译为“操作繁琐” ）