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Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.用于单纯疱疹病毒感染实验室诊断的病毒分离与感染细胞培养物的免疫染色比较。
J Clin Microbiol. 1986 Sep;24(3):487-9. doi: 10.1128/jcm.24.3.487-489.1986.
2
Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen.用于检测单纯疱疹病毒抗原的酶联免疫吸附测定法的评估
J Clin Microbiol. 1984 Jun;19(6):730-2. doi: 10.1128/jcm.19.6.730-732.1984.
3
Comparison of the Ortho Cultureset (Vero cells) and Difco Cellmatics system (mink lung and primary rabbit kidney cells) for the detection of herpes simplex virus from clinical specimens.用于从临床标本中检测单纯疱疹病毒的Ortho Cultureset（Vero细胞）和Difco Cellmatics系统（貂肺和原代兔肾细胞）的比较。
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4
Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus.用于检测单纯疱疹病毒的Cultureset与原代兔肾细胞培养的比较。
J Clin Microbiol. 1984 Jun;19(6):920-2. doi: 10.1128/jcm.19.6.920-922.1984.
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Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.细胞培养敏感性和病毒浓度对通过细胞病变效应和免疫过氧化物酶染色快速检测单纯疱疹病毒的影响。
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Comparison of cell culture with an immunoperoxidase kit for rapid diagnosis of herpes simplex virus infections.用于单纯疱疹病毒感染快速诊断的免疫过氧化物酶试剂盒与细胞培养的比较。
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Impact of cell culture sensitivity and virus concentration on rapid detection of herpes simplex virus by cytopathic effects and immunoperoxidase staining.细胞培养敏感性和病毒浓度对通过细胞病变效应和免疫过氧化物酶染色快速检测单纯疱疹病毒的影响。
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Comparison of standard culture methods, a shell vial assay, and a DNA probe for the detection of herpes simplex virus.标准培养方法、空斑试验和DNA探针检测单纯疱疹病毒的比较。
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本文引用的文献

1
Comparison of Cultureset and Bartels Immunodiagnostics with conventional tissue culture for isolation and identification of herpes simplex virus.将Cultureset和巴特尔免疫诊断法与传统组织培养法用于单纯疱疹病毒分离和鉴定的比较。
J Clin Microbiol. 1984 May;19(5):705-6. doi: 10.1128/jcm.19.5.705-706.1984.
2
Rapid detection of herpes simplex virus in clinical specimens with human embryonic lung fibroblast and primary rabbit kidney cell cultures.用人胚肺成纤维细胞和原代兔肾细胞培养物快速检测临床标本中的单纯疱疹病毒
J Clin Microbiol. 1984 Apr;19(4):563-5. doi: 10.1128/jcm.19.4.563-565.1984.
3
Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.用于检测病变标本中单纯疱疹病毒抗原的直接免疫荧光法与直接免疫过氧化物酶法的比较。
J Clin Microbiol. 1983 Aug;18(2):445-8. doi: 10.1128/jcm.18.2.445-448.1983.
4
Comparison of the Immulok cultureset kit and virus isolation for detection of herpes simplex virus in clinical specimens.Immulok培养集试剂盒与病毒分离法在临床标本中检测单纯疱疹病毒的比较。
J Clin Microbiol. 1983 Jul;18(1):222-4. doi: 10.1128/jcm.18.1.222-224.1983.
5
Comparison of cultureset to a conventional tissue culture-fluorescent-antibody technique for isolation and identification of herpes simplex virus.将培养集与传统组织培养荧光抗体技术用于单纯疱疹病毒分离和鉴定的比较。
J Clin Microbiol. 1983 Jul;18(1):215-6. doi: 10.1128/jcm.18.1.215-216.1983.
6
Comparison of guinea pig embryo cells, rabbit kidney cells, and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus.用于单纯疱疹病毒分离的豚鼠胚胎细胞、兔肾细胞和人胚肺成纤维细胞株的比较
J Clin Microbiol. 1982 May;15(5):842-7. doi: 10.1128/jcm.15.5.842-847.1982.
7
Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection.病毒分离、直接免疫荧光法和间接免疫过氧化物酶技术检测生殖器单纯疱疹病毒感染的比较
J Clin Microbiol. 1981 May;13(5):913-8. doi: 10.1128/jcm.13.5.913-918.1981.
8
Rapid detection and identification of herpes simplex virus in cell culture by a direct immunoperoxidase staining procedure.通过直接免疫过氧化物酶染色法在细胞培养中快速检测和鉴定单纯疱疹病毒。
J Clin Microbiol. 1983 Sep;18(3):550-3. doi: 10.1128/jcm.18.3.550-553.1983.
9
Comparison of Cultureset and primary rabbit kidney cell culture for the detection of herpes simplex virus.用于检测单纯疱疹病毒的Cultureset与原代兔肾细胞培养的比较。
J Clin Microbiol. 1984 Jun;19(6):920-2. doi: 10.1128/jcm.19.6.920-922.1984.
10
Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen.用于检测单纯疱疹病毒抗原的酶联免疫吸附测定法的评估
J Clin Microbiol. 1984 Jun;19(6):730-2. doi: 10.1128/jcm.19.6.730-732.1984.

用于单纯疱疹病毒感染实验室诊断的病毒分离与感染细胞培养物的免疫染色比较。

Viral isolation versus immune staining of infected cell cultures for the laboratory diagnosis of herpes simplex virus infections.

作者信息

Hughes J H, Mann D R, Hamparian V V

出版信息

J Clin Microbiol. 1986 Sep;24(3):487-9. doi: 10.1128/jcm.24.3.487-489.1986.

DOI:10.1128/jcm.24.3.487-489.1986
PMID:3020087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268946/
Abstract

The Difco Laboratories Cellmatics kit, an immune staining method for the detection of herpes simplex virus after amplification in culture, was found to be 94.6% (138 of 146) sensitive and 100% (302 of 302) specific. However, conventional culturing detected 10 (6.4%) additional viruses that were not herpes simplex. For a full-service diagnostic laboratory, viral isolation is important.

摘要

迪夫科实验室细胞分析试剂盒是一种用于在培养扩增后检测单纯疱疹病毒的免疫染色方法，结果显示其敏感性为94.6%（146例中的138例），特异性为100%（302例中的302例）。然而，传统培养法检测出另外10种（6.4%）非单纯疱疹病毒。对于提供全方位服务的诊断实验室而言，病毒分离很重要。