Genome Sequence Analysis of the Oleaginous Yeast, , and Comparison of the Carotenogenic and Oleaginous Pathway Genes and Gene Products with Other Oleaginous Yeasts.

is an oleaginous and carotenogenic yeast, useful for diverse biotechnological applications. To understand the molecular basis of its potential applications, the genome was sequenced using the Illumina MiSeq and Ion Torrent platforms, assembled by AbySS, and annotated using the JGI annotation pipeline. The genome size, 21.1 MB, was similar to that of the biotechnological "workhorse", . Comparative analyses of the genome sequence with those of other species, , , , and , were conducted, with emphasis on the carotenoid and neutral lipid biosynthesis pathways. Amino acid sequence alignments of key enzymes in the lipid biosynthesis pathway revealed why the activity of malic enzyme and ATP-citrate lyase may be ambiguous in and . Phylogenetic analysis showed a close relationship between and WP1. Dot-plot analysis of the coding sequences of the genes crtYB and ME1 corroborated sequence homologies between sequences from and . There was, however, nonsequential alignment between crtYB CDS sequences from and those from . This research presents the first genome analysis of with a focus on its biotechnological potential as a lipid and carotenoid producer.