Fakankun Irene, Fristensky Brian, Levin David B
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
J Fungi (Basel). 2021 Apr 20;7(4):320. doi: 10.3390/jof7040320.
is an oleaginous and carotenogenic yeast, useful for diverse biotechnological applications. To understand the molecular basis of its potential applications, the genome was sequenced using the Illumina MiSeq and Ion Torrent platforms, assembled by AbySS, and annotated using the JGI annotation pipeline. The genome size, 21.1 MB, was similar to that of the biotechnological "workhorse", . Comparative analyses of the genome sequence with those of other species, , , , and , were conducted, with emphasis on the carotenoid and neutral lipid biosynthesis pathways. Amino acid sequence alignments of key enzymes in the lipid biosynthesis pathway revealed why the activity of malic enzyme and ATP-citrate lyase may be ambiguous in and . Phylogenetic analysis showed a close relationship between and WP1. Dot-plot analysis of the coding sequences of the genes crtYB and ME1 corroborated sequence homologies between sequences from and . There was, however, nonsequential alignment between crtYB CDS sequences from and those from . This research presents the first genome analysis of with a focus on its biotechnological potential as a lipid and carotenoid producer.
是一种产油和产类胡萝卜素的酵母，可用于多种生物技术应用。为了解其潜在应用的分子基础，使用Illumina MiSeq和Ion Torrent平台对该基因组进行测序，由AbySS组装，并使用JGI注释管道进行注释。基因组大小为21.1 MB，与生物技术“主力军”的基因组大小相似。对该基因组序列与其他物种、、、和的基因组序列进行了比较分析，重点是类胡萝卜素和中性脂质生物合成途径。脂质生物合成途径中关键酶的氨基酸序列比对揭示了苹果酸酶和ATP-柠檬酸裂解酶在和中的活性为何可能不明确。系统发育分析表明与WP1关系密切。基因crtYB和ME1编码序列的点阵分析证实了和序列之间的序列同源性。然而，和的crtYB CDS序列之间存在非顺序比对。本研究首次对进行了基因组分析，重点关注其作为脂质和类胡萝卜素生产者的生物技术潜力。
