Department of Chemistry, National Institute of Technology, Warangal, Telangana 506004, India; Department of Energy Science and Technology, Smart Living Innovation Technology Center, Myongji University, Yongin, Gyeonggi-do 17058, Republic of Korea.
Department of Chemistry, National Institute of Technology, Warangal, Telangana 506004, India.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:93-99. doi: 10.1016/j.msec.2017.05.012. Epub 2017 May 4.
An efficient electrochemical sensor for selective detection of the neurotransmitter, epinephrine (Epn), has been fabricated with the aid of a functionalized multiwall carbon nanotube-chitosan biopolymer nanocomposite (Chit-fCNT) electrode. Multiwall carbon nanotubes (CNT) were successfully functionalized with the aid of nitric acid and confirmed by the Raman spectral data. Functionalized carbon nanotubes (fCNT) were dispersed in chitosan solution and the resulting bio-nanocomposite was used for the fabrication of sensor surface by drop and cast method. Electrochemical characteristics of the fabricated sensor were understood using cyclic, differential pulse voltammetry (CV, DPV) and electrochemical impedance analysis for the detection of Epn in phosphate buffer (pH7.4). CV and impedance analysis revealed that the Chit-fCNT modified electrode enhances the electrodic reaction of Epn and facilitated the electron transfer more readily compared to that of bare electrode. Applying DPV for the detection of Epn, achieved 30nM as the lowest detection limit in the determination range of 0.05-10μM and the analytical time as low as 10s. Selective determination of Epn against the coexistence of a number of biological electroactive interferents and reproducible results for the determination of Epn were demonstrated. The present biosensor has been found efficient for successful direct determination of Epn from pharmaceutical adrenaline formulations and urine samples.
一种高效的电化学传感器，用于选择性检测神经递质肾上腺素（Epn），是通过功能化多壁碳纳米管-壳聚糖生物聚合物纳米复合材料（Chit-fCNT）电极制备的。多壁碳纳米管（CNT）在硝酸的帮助下成功地进行了功能化，并通过拉曼光谱数据得到了证实。功能化的碳纳米管（fCNT）分散在壳聚糖溶液中，所得的生物纳米复合材料通过滴涂和浇铸法用于制备传感器表面。通过循环伏安法（CV）、差分脉冲伏安法（DPV）和电化学阻抗分析来了解所制备传感器的电化学特性，以检测磷酸盐缓冲液（pH7.4）中的 Epn。CV 和阻抗分析表明，与裸电极相比，Chit-fCNT 修饰电极增强了 Epn 的电化学反应，并更容易促进电子转移。应用 DPV 检测 Epn，在 0.05-10μM 的测定范围内，检测限低至 30nM，分析时间短至 10s。该传感器对 Epn 具有选择性，可对抗多种生物电化学干扰物的共存，并对 Epn 的测定结果具有重现性。该生物传感器已被证明可有效地直接从药物肾上腺素制剂和尿液样本中成功测定 Epn。
