Dong Zhou-Yan, Narsing Rao Manik Prabhu, Xiao Min, Wang Hong-Fei, Hozzein Wael N, Chen Wei, Li Wen-Jun
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen UniversityGuangzhou, China.
College of Life Science, Liaoning Normal UniversityDalian, China.
Front Microbiol. 2017 Jun 14;8:1090. doi: 10.3389/fmicb.2017.01090. eCollection 2017.
Diseases caused by have a significant impact on human health. We evaluated the antibacterial activity of silver nanoparticles (synthesized using the endophytic strain SYSU 333150) against . The strain SYSU 333150 was isolated from the roots of Bunge. The 16S rRNA sequence results suggest that SYSU 333150 belongs to the genus and is likely a new species. Photo-irradiation was used to synthesize silver nanoparticles, which were characterized using UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction. The nanoparticles were spherical and measured to be11 to 40 nm. X-ray diffraction revealed four peaks corresponding to the 111, 200, 220, and 311 planes of the face-centered cubic lattice, indicating a crystalline nature. Fourier transform infrared spectroscopy suggested that the metabolites in the culture supernatant were likely reducing and capping agents. The silver nanoparticles possessed antimicrobial activity (14 mm zone of inhibition) against , which was likely a result of DNA cleavage. The synthesized silver nanoparticles have potent antibacterial activity against and can be used to control infection.
由[未明确内容]引起的疾病对人类健康有重大影响。我们评估了（使用内生菌株SYSU 333150合成的）银纳米颗粒对[未明确内容]的抗菌活性。菌株SYSU 333150是从[未明确植物名称]Bunge的根部分离出来的。16S rRNA序列结果表明SYSU 333150属于[未明确属名]属，可能是一个新物种。采用光辐照法合成银纳米颗粒，并通过紫外可见光谱、透射电子显微镜和X射线衍射对其进行表征。纳米颗粒呈球形，尺寸为11至40纳米。X射线衍射显示出对应于面心立方晶格的111、200、220和311晶面的四个峰，表明其具有晶体性质。傅里叶变换红外光谱表明培养上清液中的代谢产物可能是还原剂和封端剂。银纳米颗粒对[未明确内容]具有抗菌活性（抑菌圈直径为14毫米），这可能是DNA裂解的结果。合成的银纳米颗粒对[未明确内容]具有强大的抗菌活性，可用于控制感染。
