Lu Bing, Zhou Haijian, Zhang Xin, Qu Mei, Huang Ying, Wang Quanyi
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing Center for Prevention Medical Research, Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing, 100013 China.
State Key Laboratory for Infection Disease Prevention and Control, National Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, 102206 China.
Gut Pathog. 2017 Jun 30;9:39. doi: 10.1186/s13099-017-0188-7. eCollection 2017.
() is an opportunistic pathogen associated with community-acquired infections and nosocomial infections. From 2010 to 2015, testing was included into the exiting diarrhea-syndrome surveillance with objective to estimate the prevalence of in diarrhea-syndrome patients, test antibiotics susceptibility and investigate molecular characteristics.
Stool specimens from diarrhea-syndrome outpatients were cultured and identified the pathogens by the Vitek2 Compact instrument. The isolated strains were tested for antibiotics susceptibility by minimal inhibitory concentration (MIC) method, and subtyped by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).
22 strains were identified from 4340 stool specimens of outpatients who visited sentinel hospitals in Beijing during 2010-2015. All strains were sensitive to gentamicin, nalidixic acid, ciprofloxacin, ceftriaxone, cefotaxime, cefepime, imipenem. The highest resistance rate of strains was 100% to amoxicillin-clavulanate, followed by 72.7% to ampicillin. These 22 strains were characterized into 21 different PFGE types and 20 MLST types with less similarity.
The detection rate of in stool specimens from outpatients with diarrhea syndromes was about 0.5% in Beijing. Less similarity of the isolated strains indicated the unlikely long-term circulating of in the community. ST23 was the most common genotype. Drug resistance of the community-acquired was not a serious problem in comparing with hospital-acquired infections. High vigilance in the community-acquired strains and investigation of pathogens' microbiological characteristics are valuable for signals detection for drug resistance in population and strains variation.
（）是一种与社区获得性感染和医院感染相关的机会性病原体。2010年至2015年，检测被纳入现有的腹泻综合征监测，目的是估计腹泻综合征患者中（）的流行率，检测抗生素敏感性并研究分子特征。
对腹泻综合征门诊患者的粪便标本进行培养，并用Vitek2 Compact仪器鉴定病原体。对分离出的（）菌株采用最低抑菌浓度（MIC）法检测抗生素敏感性，并用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）进行分型。
2010 - 2015年期间，在北京定点医院就诊的门诊患者的4340份粪便标本中鉴定出22株（）菌株。所有菌株对庆大霉素、萘啶酸、环丙沙星、头孢曲松、头孢噻肟、头孢吡肟、亚胺培南敏感。（）菌株对阿莫西林 - 克拉维酸的耐药率最高，为100%，其次对氨苄西林的耐药率为72.7%。这22株（）菌株被分为21种不同的PFGE型和20种MLST型，相似度较低。
在北京，腹泻综合征门诊患者粪便标本中（）的检出率约为0.5%。分离菌株相似度较低表明该菌在社区不太可能长期循环传播。ST23是最常见的基因型。与医院获得性感染相比，社区获得性（）的耐药性不是一个严重问题。对社区获得性（）菌株保持高度警惕并调查病原体的微生物学特征，对于人群耐药性信号检测和菌株变异研究具有重要价值。
