超重多囊卵巢综合征患者体重指数与卵泡液基因表达及维生素D水平之间关系的评估
Evaluation of Relationship between Body Mass Index with Gene Expression and Vitamin D Levels of Follicular Fluid in Overweight Patients with Polycystic Ovary Syndrome.
作者信息
Aghadavod Esmat, Mollaei Hakimeh, Nouri Mohammad, Hamishehkar Hamed
机构信息
Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Int J Fertil Steril. 2017 Jul-Sep;11(2):105-111. doi: 10.22074/ijfs.2017.4704. Epub 2017 Feb 16.
BACKGROUND
Polycystic ovary syndrome (PCOS) is the most common endocrine disorder associated with reproductive disorders and metabolic dysfunctions including insulin resistance. The roles of vitamin D in the regulation of metabolic modulations specifically involving insulin and reproduction processing are introduced. In addition, obesity appears to be closely associated with severity of PCOS. The present study is to evaluate the effect of body mass index (BMI) on vitamin D levels in follicular fluid and expression levels in granulos cells.
MATERIALS AND METHODS
A comparative study was conducted on 80 women with average age of 20-35 years referred for fertilization (IVF). Patients were divided into four groups, and serum levels of testosterone and insulin resistance (IR) were evaluated at the puncture time. Also, vitamin D levels of follicular fluid were evaluated. gene expression was assayed by quantified-polymerase chain reaction (PCR) technique. Correlations were evaluated with calculation of the Spearman coefficient, and also independent relationships were assessed by means of multiple regression analysis.
RESULTS
Vitamin D levels of follicular fluid decreased in PCOS patients compared with non-PCOS. Also, over-weight individuals had lower vitamin D levels compared with normal-weight patients. Vitamin D levels of follicular fluid were highly correlated with BMI (r=-0.51, P<0.01). Homeostatic model assessment-IR (HOMA-IR) values were significantly higher in women of PCOS/overweight and PCOS/normal weight in comparison with women of non-PCOS/normal weight (P<0.01). The gene expression data of in granulosa cells were significantly lower in the PCOS/overweight group compared with the non-PCOS/normal weight (P<0.01).
CONCLUSION
The findings indicated significant differences in gene expression in granulosa cells and vitamin D of follicular fluid in PCOS/overweight patients.
背景
多囊卵巢综合征（PCOS）是与生殖障碍和包括胰岛素抵抗在内的代谢功能障碍相关的最常见内分泌疾病。介绍了维生素D在调节特别是涉及胰岛素和生殖过程的代谢调节中的作用。此外，肥胖似乎与PCOS的严重程度密切相关。本研究旨在评估体重指数（BMI）对卵泡液中维生素D水平和颗粒细胞中表达水平的影响。
材料与方法
对80名平均年龄为20 - 35岁前来接受体外受精（IVF）的女性进行了一项比较研究。将患者分为四组，并在穿刺时评估睾酮血清水平和胰岛素抵抗（IR）。同时，评估卵泡液中的维生素D水平。通过定量聚合酶链反应（PCR）技术检测基因表达。通过计算Spearman系数评估相关性，并通过多元回归分析评估独立关系。
结果
与非PCOS患者相比，PCOS患者卵泡液中的维生素D水平降低。此外，超重个体的维生素D水平低于正常体重患者。卵泡液中的维生素D水平与BMI高度相关（r = -0.51，P < 0.01）。与非PCOS/正常体重女性相比，PCOS/超重和PCOS/正常体重女性的稳态模型评估-IR（HOMA-IR）值显著更高（P < 0.01）。与非PCOS/正常体重组相比，PCOS/超重组颗粒细胞中的基因表达数据显著更低（P < 0.01）。
结论
研究结果表明，PCOS/超重患者颗粒细胞中的基因表达和卵泡液中的维生素D存在显著差异。
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