Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut, Messeweg 11-12, 38104 Braunschweig, Germany.
New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland, New Zealand.
Curr Opin Virol. 2017 Oct;26:8-14. doi: 10.1016/j.coviro.2017.06.010. Epub 2017 Jul 22.
Mild strain cross-protection is currently an important method for the production of high quality plant products; despite challenge from severe virus isolates the initial protecting strain precludes symptom development. The mechanism of cross-protection is not yet resolved as RNA silencing does not sufficiently explain the phenomenon. Six requirements have been put forward to ensure long-lasting protection. We propose two additional requirements for effective and durable mild strain cross-protection; mild strains based on knowledge of the mechanism and consideration of impacts to consumers. Future research on predicting phenotype from genotype and understanding virus-plant and virus-vector interactions will enable improvement of cross-protective strains. Shared international databases of whole ecosystem interactions across a wide range of virus patho- and symbiotic-systems will form the basis for making step-change advances towards our collective ability to engineer and improve mild strain cross-protection.
轻度应变交叉保护目前是生产高质量植物产品的重要方法；尽管受到严重病毒分离株的挑战，但最初的保护株可防止症状的发展。交叉保护的机制尚未解决，因为 RNA 沉默并不能充分解释这一现象。为确保长期保护，已经提出了六个要求。我们提出了另外两个要求，以确保有效和持久的轻度应变交叉保护；基于对机制的了解和对消费者影响的考虑，基于轻度应变的保护。对从基因型预测表型和理解病毒-植物和病毒-载体相互作用的未来研究，将使交叉保护株得到改善。共享广泛的病毒病理和共生系统的整个生态系统相互作用的国际数据库，将为朝着我们集体设计和改善轻度应变交叉保护的能力迈出一大步奠定基础。
