Karygianni Lamprini, Jähnig Andrea, Schienle Stefanie, Bernsmann Falk, Adolfsson Erik, Kohal Ralf J, Chevalier Jérôme, Hellwig Elmar, Al-Ahmad Ali
Division of Restorative Dentistry and Periodontology, Department of Dental Medicine, Oral and Maxillofacial Surgery, Medical Center, University of Freiburg, Hugstetter Str. 55, Freiburg 79106, Germany.
NTTF Coatings GmbH, Maarweg 32, Rheinbreitbach 53619, Germany.
Materials (Basel). 2013 Dec 4;6(12):5659-5674. doi: 10.3390/ma6125659.
Bacterial adhesion to implant biomaterials constitutes a virulence factor leading to biofilm formation, infection and treatment failure. The aim of this study was to examine the initial bacterial adhesion on different implant materials . Four implant biomaterials were incubated with , and for 2 h: 3 mol % yttria-stabilized tetragonal zirconia polycrystal surface (B1a), B1a with zirconium oxide (ZrO₂) coating (B2a), B1a with zirconia-based composite coating (B1b) and B1a with zirconia-based composite and ZrO₂ coatings (B2b). Bovine enamel slabs (BES) served as control. The adherent microorganisms were quantified and visualized using scanning electron microscopy (SEM); DAPI and live/dead staining. The lowest bacterial count of was detected on BES and the highest on B1a. The fewest vital strains (42.22%) were detected on B2a surfaces, while most and strains (approximately 80%) were vital overall. Compared to BES; coated and uncoated zirconia substrata exhibited no anti-adhesive properties. Further improvement of the material surface characteristics is essential.
细菌对植入生物材料的粘附构成一种毒力因子，可导致生物膜形成、感染及治疗失败。本研究旨在检测不同植入材料上细菌的初始粘附情况。将四种植入生物材料与[具体物质未给出]一起孵育2小时：3摩尔%氧化钇稳定四方多晶氧化锆表面（B1a）、涂有氧化锆（ZrO₂）涂层的B1a（B2a）、涂有氧化锆基复合涂层的B1a（B1b）以及涂有氧化锆基复合涂层和ZrO₂涂层的B1a（B2b）。牛牙釉质板（BES）作为对照。使用扫描电子显微镜（SEM）、DAPI和活/死染色对粘附的微生物进行定量和可视化。在BES上检测到的细菌数量最少，在B1a上最多。在B2a表面检测到的活[具体细菌未给出]菌株最少（42.22%），而总体上大多数[具体细菌未给出]和[具体细菌未给出]菌株（约80%）是活的。与BES相比，涂覆和未涂覆的氧化锆基质均未表现出抗粘附特性。进一步改善材料表面特性至关重要。
