Nganga Sara, Travan Andrea, Marsich Eleonora, Donati Ivan, Söderling Eva, Moritz Niko, Paoletti Sergio, Vallittu Pekka K
Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Turku, Finland,
J Mater Sci Mater Med. 2013 Dec;24(12):2775-85. doi: 10.1007/s10856-013-5022-2. Epub 2013 Aug 7.
Biostable fiber-reinforced composite (FRC) implants prepared from bisphenol-A-dimethacrylate and triethyleneglycoldimethacrylate resin reinforced with E-glass fibers have been successfully used in cranial reconstructions in 15 patients. Recently, porous FRC structures were suggested as potential implant materials. Compared with smooth surface, porous surface allows implant incorporation via bone ingrowth, but is also a subject to bacterial attachment. Non-cytotoxic silver-polysaccharide nanocomposite coatings may provide a way to decrease the risk of bacterial contamination of porous FRC structures. This study is focused on the in vitro characterization of the effect porosity on the antimicrobial efficiency of the coatings against Staphylococcus aureus and Pseudomonas aeruginosa by a series of microbiological tests (initial adhesion, antimicrobial efficacy, and biofilm formation). Characterization included confocal laser scanning microscopy and scanning electron microscopy. The effect of porosity on the initial attachment of S. aureus was pronounced, but in the case of P. aeruginosa the effect was negligible. There were no significant effects of the coatings on the initial bacterial attachment. In the antimicrobial efficacy test, the coatings were potent against both strains regardless of the sample morphology. In the biofilm tests, there were no clear effects either of morphology or of the coating. Further coating development is foreseen to achieve a longer-term antimicrobial effect to inhibiting bacterial implant colonization.
由双酚 A - 二甲基丙烯酸酯和三乙二醇二甲基丙烯酸酯树脂与 E 玻璃纤维增强制成的生物稳定纤维增强复合材料（FRC）植入物已成功应用于 15 例患者的颅骨重建。最近，多孔 FRC 结构被认为是潜在的植入材料。与光滑表面相比，多孔表面允许通过骨长入实现植入物整合，但也容易附着细菌。无细胞毒性的银 - 多糖纳米复合涂层可能提供一种降低多孔 FRC 结构细菌污染风险的方法。本研究通过一系列微生物学测试（初始粘附、抗菌效果和生物膜形成），重点对孔隙率对涂层针对金黄色葡萄球菌和铜绿假单胞菌的抗菌效率的体外特性进行表征。表征包括共聚焦激光扫描显微镜和扫描电子显微镜。孔隙率对金黄色葡萄球菌的初始附着影响显著，但对铜绿假单胞菌的影响可忽略不计。涂层对细菌的初始附着没有显著影响。在抗菌效果测试中，无论样品形态如何，涂层对两种菌株均有效。在生物膜测试中，形态和涂层均未产生明显影响。预计进一步开发涂层以实现抑制细菌植入物定植的长期抗菌效果。
