Tanaka Reiji, Cleenwerck Ilse, Mizutani Yukino, Iehata Shunpei, Bossier Peter, Vandamme Peter
Laboratory of Marine Microbiology, Mie University, Kurima-machiya 1577, Tsu, Mie, 514-8507, Japan.
BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Faculty of Science, Ghent University, Campus Ledeganck, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3050-3056. doi: 10.1099/ijsem.0.002080. Epub 2017 Aug 18.
A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5T, was isolated from the gut of an abalone of the species Haliotis gigantea collected in Japan. Phylogenetic analyses based on 16S rRNA, gyrB, hsp60 and rpoB gene sequences placed strain MA5T in the genus Arcobacter in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established Arcobacter species revealed A. nitrofigilis (95.1 %) as nearest neighbour. Strain MA5T grew optimally at 25 °C, pH 6.0 to 9.0 and in the presence of 2 to 5 % (w/v) NaCl under both aerobic and microaerobic conditions. The predominant fatty acids found were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c), C12 : 0 3-OH and C18 : 1 ω7c. Menaquinone-6 (MK-6) and menaquinone-7 (MK-7) were found as the major respiratory quinones. The major polar lipids detected were phosphatidylethanolamine and phosphatidylglycerol. Strain MA5T could be differentiated phenotypically from the phylogenetic closest Arcobacter species by its ability to grow on 0.05 % safranin and 0.01 % 2,3,5-triphenyl tetrazolium chloride (TTC), but not on 0.5 % NaCl. The obtained DNA G+C content of strain MA5T was 27.9 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic distinctiveness of MA5T, this strain is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter haliotis sp. nov. is proposed. The type strain is MA5T (=LMG 28652T=JCM 31147T).
从日本采集的巨大鲍的肠道中分离出一株革兰氏阴性、需氧、具极鞭毛的杆状细菌，有时略弯曲，命名为MA5T。基于16S rRNA、gyrB、hsp60和rpoB基因序列的系统发育分析将菌株MA5T置于弓形杆菌属的一个独立系统发育分支中。将该菌株的16S rRNA基因序列与已确定的弓形杆菌属模式菌株的序列进行比较，发现嗜硝化弓形杆菌（95.1%）为最近邻。菌株MA5T在25℃、pH 6.0至9.0以及2%至5%（w/v）NaCl存在的条件下，在需氧和微需氧条件下均能最佳生长。发现的主要脂肪酸为总特征3（异-C15:0 2-OH和/或C16:1 ω7c）、C12:0 3-OH和C18:1 ω7c。主要呼吸醌为甲基萘醌-6（MK-6）和甲基萘醌-7（MK-7）。检测到的主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。菌株MA5T可通过在0.05%番红和0.01% 2,3,5-三苯基氯化四氮唑（TTC）上生长，但不在0.5% NaCl上生长的能力，从系统发育关系最密切的弓形杆菌属物种中进行表型区分。菌株MA5T的DNA G+C含量为27.9 mol%。基于MA5T的系统发育、化学分类和表型独特性，该菌株被认为代表弓形杆菌属的一个新物种，为此提出新名称嗜鲍弓形杆菌（Arcobacter haliotis sp. nov.）。模式菌株为MA5T（=LMG 28652T=JCM 31147T）。
