Department of Chemistry & Institute of Biomedicine Science & State Key Laboratory of Molecular Engineering of Polymers & Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , 220 Handan Road, Shanghai 200433, P. R. China.
ACS Appl Mater Interfaces. 2017 Sep 27;9(38):32583-32590. doi: 10.1021/acsami.7b10618. Epub 2017 Sep 14.
Persistent luminescence (PL) bioimaging provides an optimal method of eliminating autofluorescence for a higher resolution and sensitivity because of the absence of excitation light. However, ultraviolet light is still necessary in common energy charging processes, which limits its reactivation in vivo because of its low penetration depth. In the present study, we introduce a type of hybrid nanocluster (UCPL-NC) composed of upconversion nanoparticles, β-NaYbF:Tm@NaYF, and persistent nanoparticles, ZnGaGeO:0.5%Cr, which can be activated by a 980 nm laser and exhibits an afterglow at 700 nm to realize near-infrared (NIR) to NIR UCPL bioimaging. The PL of the UCPL-NCs can be reactivated even when covered with a 10 mm pork. We demonstrate that these polyethylene glycol-modified phospholipid-functionalized UCPL-NCs can be reactivated in vivo and applied in the PL lymphatic imaging on small animals.
持续发光（PL）生物成像提供了一种消除自发荧光的最佳方法，因为它没有激发光，因此具有更高的分辨率和灵敏度。然而，在常见的能量充电过程中仍然需要紫外光，这限制了其在体内的再激活，因为它的穿透深度较低。在本研究中，我们引入了一种由上转换纳米粒子β-NaYbF:Tm@NaYF 和持久发光纳米粒子 ZnGaGeO:0.5%Cr 组成的混合纳米簇（UCPL-NC），它可以被 980nm 激光激活，并在 700nm 处发出余晖，从而实现近红外（NIR）到近红外的 UCPL 生物成像。即使覆盖了 10mm 的猪肉，UCPL-NC 的 PL 也可以被重新激活。我们证明了这些经过聚乙二醇修饰的磷脂功能化 UCPL-NC 可以在体内被重新激活，并应用于小动物的 PL 淋巴成像。
