Regenerative Biomaterials Group, RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, HA6 2RN, UK.
Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
Macromol Biosci. 2017 Nov;17(11). doi: 10.1002/mabi.201700185. Epub 2017 Sep 12.
The aim of this study is to design a novel two-component hybrid scaffold using the fibrin/alginate porous hydrogel Smart Matrix combined to a backing layer of plasma polymerized polydimethylsiloxane (Sil) membrane to make the fibrin-based dermal scaffold more robust for the treatment of the clinically challenging pressure sores. A design criteria are established, according to which the Sil membranes are punched to avoid collection of fluid underneath. Manual peel test shows that native silicone does not attach to the fibrin/alginate component while the plasma polymerized silicone membranes are firmly bound to fibrin/alginate. Structural characterization shows that the fibrin/alginate matrix is intact after the addition of the Sil membrane. By adding a Sil membrane to the original fibrin/alginate scaffold, the resulting two-component scaffolds have a significantly higher shear or storage modulus G'. In vitro cell studies show that dermal fibroblasts remain viable, proliferate, and infiltrate the two-component hybrid scaffolds during the culture period. These results show that the design of a novel two-component hybrid dermal scaffold is successful according to the proposed design criteria. To the best of the authors' knowledge, this is the first study that reports the combination of a fibrin-based scaffold with a plasma-polymerized silicone membrane.
本研究旨在设计一种新型的两组件混合支架，使用纤维蛋白/海藻酸钠多孔水凝胶 Smart Matrix 与等离子体聚合聚二甲基硅氧烷 (Sil) 膜的背衬层相结合，使基于纤维蛋白的真皮支架更坚固，以治疗临床上具有挑战性的压疮。根据设计标准，Sil 膜被打孔以避免在其下方积液。手动剥离试验表明，天然硅酮不会附着在纤维蛋白/海藻酸盐成分上，而等离子体聚合的硅酮膜则牢固地结合在纤维蛋白/海藻酸盐上。结构特征表明，在添加 Sil 膜后，纤维蛋白/海藻酸盐基质保持完整。通过在原始纤维蛋白/海藻酸盐支架上添加 Sil 膜，得到的两组件支架的剪切或储能模量 G'显著增加。体外细胞研究表明，在培养期间，真皮成纤维细胞保持存活、增殖并渗透到两组件混合支架中。这些结果表明，根据提出的设计标准，新型两组件混合真皮支架的设计是成功的。据作者所知，这是首次报道将基于纤维蛋白的支架与等离子体聚合的硅酮膜结合的研究。
