School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, PR China.
Department of Zoology and Fisheries, University of Agriculture, Faisalabad 38000, Pakistan.
Int J Biol Macromol. 2018 Feb;107(Pt A):1142-1150. doi: 10.1016/j.ijbiomac.2017.09.093. Epub 2017 Sep 28.
In the present study, we sequenced the entire mitochondrial genome (mitogenome) of Ectropis oblique using PCR amplification and sequencing methods The entire mitogenome is 15,356bp long, including 13 protein coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes and A+T rich element. The base composition and gene arrangement are identical to those of other lepidopterans. Of these 37 genes, twenty three are resided on heavy strand, while fourteen located on light strand. The newly sequenced mitogenome displayed a biased Adenine/thymine 84.94% usage versus guanine/cytosine. The AT skewness is 0.030 and the GC skewness is -0.180. Twelve out of 13 PCGs initiated with canonical start codon (ATN), while cox1 started with CGA. The A+T rich element of E. oblique is 363bp long and contains of many features common to lepidopteran insects, including the 'ATAGA' motif, a 22bp poly (T) stretch and a microsatellite-like (AT) element upstream of trnM. Further, this mitogenome comprises 15 intergenic spacer and overlapping regions. Phylogenetic analyses showed that E. oblique belongs to Geometridae, and that the monophyly of Lepidoptera superfamilies is well supported.
在本研究中，我们采用 PCR 扩增和测序方法对斜纹夜蛾的整个线粒体基因组（mitogenome）进行了测序。整个 mitogenome 长 15356bp，包括 13 个蛋白质编码基因、22 个转移 RNA（tRNA）基因、两个核糖体 RNA（rRNA）基因和 A+T 丰富元件。碱基组成和基因排列与其他鳞翅目昆虫相同。在这 37 个基因中，有 23 个位于重链上，而 14 个位于轻链上。新测序的 mitogenome 显示出偏向腺嘌呤/胸腺嘧啶 84.94%的使用，而鸟嘌呤/胞嘧啶为 15.06%。AT 偏斜为 0.030，GC 偏斜为-0.180。13 个 PCGs 中有 12 个以典型的起始密码子（ATN）起始，而 cox1 以 CGA 起始。斜纹夜蛾的 A+T 丰富元件长 363bp，包含许多鳞翅目昆虫共有的特征，包括“ATAGA”基序、22bp 的多（T）延伸和 trnM 上游的微卫星样（AT）元件。此外，这个 mitogenome 包括 15 个基因间间隔区和重叠区。系统发育分析表明，斜纹夜蛾属于夜蛾科，鳞翅目超科的单系性得到了很好的支持。
