Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, the Third Military Medical University, Chongqing, 400038, China.
Chongqing Key Laboratory for Disease Proteomics, Chongqing, 400038, China.
Sci Rep. 2017 Mar 27;7(1):436. doi: 10.1038/s41598-017-00594-x.
An ideal wound dressing can both promote wound healing and prevent bacterial infection. Here, we report a potential dressing prepared by incorporating an optimized concentration of silver nanoparticles (AgNPs) into the microfibers of a natural eggshell membrane (EM) using environmentally friendly and mussel-inspired dopamine. Briefly, acid-treated EM was used as a porous membrane for polydopamine-reduced AgNPs synthesis. To obtain the optimal cytocompatible silver concentration, cellular attachment and MTT assay were performed with different concentrations of AgNPs. The morphology of the EM and AgNPs was confirmed by scanning electronic microscopy, scanning transmission electronic microscopy and Fourier transform infrared spectroscopy. The synthesized EM/AgNPs exhibited steady and safe AgNPs release, which was further tested for antibacterial activity against Escherichia coli and Staphylococcus aureus by disc diffusion method and bacterial suspension assay. Finally, in a murine full-thickness skin wound model, we found that EM/AgNPs could promote re-epithelialization, granulation tissue formation and wound healing via enhancing cell proliferation, as demonstrated by the expression of proliferating cell nuclear antigen (PCNA), and controlling inflammation response, as demonstrated by the expression of interleukin-1β (IL-1β). These findings suggest that EM/AgNPs may have a promising application in wound management.
一种理想的创伤敷料既能促进伤口愈合，又能防止细菌感染。在这里，我们报告了一种潜在的敷料，它是通过将优化浓度的银纳米粒子（AgNPs）掺入到天然蛋壳膜（EM）的微纤维中，利用环保且受贻贝启发的多巴胺制备而成。简要地说，经过酸处理的 EM 被用作多孔膜，用于合成聚多巴胺还原的 AgNPs。为了获得最佳细胞相容性的银浓度，通过不同浓度的 AgNPs 进行了细胞附着和 MTT 测定。通过扫描电子显微镜、扫描透射电子显微镜和傅里叶变换红外光谱确认了 EM 和 AgNPs 的形态。合成的 EM/AgNPs 表现出稳定和安全的 AgNPs 释放，进一步通过圆盘扩散法和细菌悬浮液测定法测试了其对大肠杆菌和金黄色葡萄球菌的抗菌活性。最后，在小鼠全层皮肤创伤模型中，我们发现 EM/AgNPs 可以通过增强细胞增殖（如增殖细胞核抗原（PCNA）的表达所示）促进再上皮化、肉芽组织形成和伤口愈合，并通过控制炎症反应（如白细胞介素-1β（IL-1β）的表达所示）。这些发现表明 EM/AgNPs 在伤口管理中可能有很好的应用前景。
