Safarpoor Dehkordi Farhad, Tirgir Farhang, Valizadeh Yousef
Young Researchers and Elites Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Vet Res Forum. 2017 Summer;8(3):215-221. Epub 2017 Sep 15.
Application of smoke condensate derived from an indirect heating of jennet feces (Sargin) had been recommended by Iranian ancient scientists as a therapeutic agent. The present study was done to evaluate the healing effects of Guajol ointment on burn wound in rat. The Guajol ointment was prepared from the smoke condensate of Sargin samples. Wistar Rats (n = 50) were randomized into six groups including normal saline, silver sulfadiazine and 1.25%, 2.50%, 5.00% and 10.00% concentrations of Guajol ointment. Under general anesthesia, dorsum of the rats were shaved and burn wounds were created using hot plate. Area of wounds and percent of healing were measured. Normal saline had the highest area of wound, followed by 1.25% Guajol and silver-sulfadiazine groups. The group treated with 5.00% Guajol showed the highest percent of healing. Percent of healing in NS, SSD and 5.00% Guajol ointment groups on day 21 were 38.47%, 75.00% and 98.51%, respectively. Microscopic examination of wounds sections of rats treated with 5.00% Guajol showed more collagen fibers and fibroblasts cells on day 7. Wounds of 5.00% Guajol treated group was covered with healthy epithelial and epidermis tissues and hair follicles on day 21. This was the first report of using Sargin to heal the burn wound of rat. Further studies are recommended for investigation of the other effects of Guajol ointment and its possible application in medicine.
伊朗古代科学家曾推荐将间接加热驴粪（萨尔金）产生的烟雾冷凝物用作治疗剂。本研究旨在评估瓜焦尔软膏对大鼠烧伤创面的愈合效果。瓜焦尔软膏由萨尔金样品的烟雾冷凝物制备而成。将50只Wistar大鼠随机分为六组，包括生理盐水组、磺胺嘧啶银组以及浓度为1.25%、2.50%、5.00%和10.00%的瓜焦尔软膏组。在全身麻醉下，剃去大鼠背部毛发，使用热板造成烧伤创面。测量创面面积和愈合百分比。生理盐水组的创面面积最大，其次是1.25%瓜焦尔软膏组和磺胺嘧啶银组。5.00%瓜焦尔软膏治疗组的愈合百分比最高。在第21天，生理盐水组、磺胺嘧啶银组和5.00%瓜焦尔软膏组的愈合百分比分别为38.47%、75.00%和98.51%。对5.00%瓜焦尔软膏治疗的大鼠创面切片进行显微镜检查，结果显示在第7天时胶原纤维和成纤维细胞更多。在第21天，5.00%瓜焦尔软膏治疗组的创面覆盖有健康的上皮和表皮组织以及毛囊。这是关于使用萨尔金治疗大鼠烧伤创面的首次报道。建议进一步研究瓜焦尔软膏的其他作用及其在医学上的可能应用。
