Department of Genetics and Genomic Sciences, Icahn Institute for Genomics and Multiscale Biology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Division of Pediatric Allergy and Immunology, Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Allergy. 2018 Jul;73(7):1515-1524. doi: 10.1111/all.13389. Epub 2018 Mar 15.
Gut microbiota may play a role in egg allergy. We sought to examine the association between early-life gut microbiota and egg allergy.
We studied 141 children with egg allergy and controls from the multicenter Consortium of Food Allergy Research study. At enrollment (age 3 to 16 months), fecal samples were collected, and clinical evaluation, egg-specific IgE measurement, and egg skin prick test were performed. Gut microbiome was profiled by 16S rRNA sequencing. Analyses for the primary outcome of egg allergy at enrollment, and the secondary outcomes of egg sensitization at enrollment and resolution of egg allergy by age 8 years, were performed using Quantitative Insights into Microbial Ecology, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States, and Statistical Analysis of Metagenomic Profiles.
Compared to controls, increased alpha diversity and distinct taxa (PERMANOVA P = 5.0 × 10 ) characterized the early-life gut microbiome of children with egg allergy. Genera from the Lachnospiraceae, Streptococcaceae, and Leuconostocaceae families were differentially abundant in children with egg allergy. Predicted metagenome functional analyses showed differential purine metabolism by the gut microbiota of egg-allergic subjects (Kruskal-Wallis P = 0.021). Greater gut microbiome diversity and genera from Lachnospiraceae and Ruminococcaceae were associated with egg sensitization (PERMANOVA P = 5.0 × 10 ). Among those with egg allergy, there was no association between early-life gut microbiota and egg allergy resolution by age 8 years.
The distinct early-life gut microbiota in egg-allergic and egg-sensitized children identified by our study may point to targets for preventive or therapeutic intervention.
肠道微生物群可能在鸡蛋过敏中发挥作用。我们试图研究生命早期肠道微生物群与鸡蛋过敏之间的关联。
我们研究了来自多中心食物过敏研究联盟的 141 名鸡蛋过敏儿童和对照者。在入组时（3 至 16 个月），收集粪便样本，并进行临床评估、鸡蛋特异性 IgE 测量和鸡蛋皮试。通过 16S rRNA 测序对肠道微生物组进行分析。使用定量微生物生态学、群落重建的系统发育分析和宏基因组分析的统计方法，对入组时鸡蛋过敏的主要结局以及入组时鸡蛋致敏和 8 岁时鸡蛋过敏缓解的次要结局进行分析。
与对照组相比，鸡蛋过敏儿童的生命早期肠道微生物群具有较高的 alpha 多样性和独特的分类群（PERMANOVA P=5.0×10）。鸡蛋过敏儿童的 Lachnospiraceae、Streptococcaceae 和 Leuconostocaceae 属的丰度存在差异。预测的宏基因组功能分析表明，鸡蛋过敏受试者的肠道微生物群具有不同的嘌呤代谢（Kruskal-Wallis P=0.021）。肠道微生物群多样性较高，Lachnospiraceae 和 Ruminococcaceae 属丰度较高与鸡蛋致敏有关（PERMANOVA P=5.0×10）。在有鸡蛋过敏的儿童中，生命早期肠道微生物群与 8 岁时鸡蛋过敏缓解之间没有关联。
我们的研究确定了鸡蛋过敏和鸡蛋致敏儿童的独特生命早期肠道微生物群，这可能为预防或治疗干预提供了靶点。
